비타민B2 (리보플라빈)

Vitamin B2 (riboflavin) and a mixture of vitamin B2 and C affects MMC efficiency in aerated media under irradiation.

1. Anticancer Res. 2004 Nov-Dec;24(6):4031-4. Vitamin B2 (riboflavin) and a mixture of vitamin B2 and C affects MMC efficiency in aerated media under irradiation. Fuga L(1), Kragl M, Getoff N. Author information: (1)Ludwig Boltzmann Institute for Radiation Chemistry and Radiation Biology, A-109

Potentiation by amino acid of the therapeutic effect of highly purified vitamin B2 in mice with lipopolysaccharide-induced shock.

2. Eur J Pharmacol. 2004 Jun 16;493(1-3):177-82. doi: 10.1016/j.ejphar.2004.04.019. Potentiation by amino acid of the therapeutic effect of highly purified vitamin B2 in mice with lipopolysaccharide-induced shock. Toyosawa T(1), Suzuki M, Kodama K, Araki S. Author information: (1)Tsukuba Researc

Is thallium-induced nephrotoxicity in rats connected with riboflavin and/or GSH?--reconsideration of hypotheses on the mechanism of thallium toxicity.

3. J Appl Toxicol. 1999 Jan-Feb;19(1):61-6. doi: 10.1002/(sici)1099-1263(199901/02)19:1<61::aid-jat539>3.0.co;2-s. Is thallium-induced nephrotoxicity in rats connected with riboflavin and/or GSH?--reconsideration of hypotheses on the mechanism of thallium toxicity. Appenroth D(1), Winnefeld K.

Potentiation of sodium chromate(VI)-induced chromosomal aberrations and mutation by vitamin B2 in Chinese hamster V79 cells.

4. Mutat Res. 1992 Nov;283(3):211-4. doi: 10.1016/0165-7992(92)90109-u. Potentiation of sodium chromate(VI)-induced chromosomal aberrations and mutation by vitamin B2 in Chinese hamster V79 cells. Sugiyama M(1), Tsuzuki K, Lin X, Costa M. Author information: (1)Department of Medical Biochemistry

Assessment of vitamin A, vitamin B(2), vitamin B(12), vitamin K, folate, and choline status following 4 months of multinutrient supplementation in healthy vegans: a randomised, double-blind, placebo-controlled trial.

1. Eur J Nutr. 2025 Dec 19;65(1):9. doi: 10.1007/s00394-025-03814-7. Assessment of vitamin A, vitamin B(2), vitamin B(12), vitamin K, folate, and choline status following 4 months of multinutrient supplementation in healthy vegans: a randomised, double-blind, placebo-controlled trial. Zerback T(1), Koeder C(2)(3), Weder S(1), Sputtek A(4), Eckert GP(5), Keller M(1). Author information: (1)Research Institute for Plant-Based Nutrition, 35444, Biebertal, Germany. (2)Institute for Prevention and Cancer Epidemiology (IPE), Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, 79110, Freiburg im Breisgau, Germany. christiankoeder@gmail.com. (3)Research Institute for Plant-Based Nutrition, 35444, Biebertal, Germany. christiankoeder@gmail.com. (4)MVZ Medical Laboratory Bremen GmbH, 28359, Bremen, Germany. (5)Institute of Nutritional Sciences, Justus-Liebig University, 35392, Giessen, Germany. PURPOSE: The aim of the MultiVeg study, a double-blind, randomised controlled trial (RCT), was to investigate the nutritional status of healthy vegans following 4 months of multinutrient supplementation. METHODS: A double-blind, RCT was conducted with 72 vegan adults (19-57 years) in Germany. Data on anthropometric parameters, dietary nutrient intake, and nutritional status were collected. The nutritional status of the participants was assessed at baseline and after 4 months. The results were compared between groups using ANCOVA. The results for vitamins and choline are presented here. RESULTS: After adjustment for baseline values, age, sex, and multiple testing, no significant between-group differences in biomarker concentration changes from baseline to 4 months were observed for vitamin A, retinol-binding protein, transthyretin, beta-carotene, methylmalonic acid, homocysteine, choline, total osteocalcin, carboxylated and undercarboxylated osteocalcin, and folate. In contrast, significant between-group differences in changes were observed for flavin adenine dinucleotide (FAD), serum vitamin B12, holotranscobalamin, and the combined vitamin B12 status indicator (cB12) after adjustment. CONCLUSION: A multinutrient supplement containing 82 µg of vitamin B12 per day significantly positively affected vitamin B12 blood biomarkers in healthy vegans. REGISTRATION: This study was registered in the German Clinical Trials Register (DRKS00028151). © 2025. The Author(s). DOI: 10.1007/s00394-025-03814-7 PMCID: PMC12717231 PMID: 41417236 [Indexed for MEDLINE] Conflict of interest statement: Declarations. Conflict of interest: T.Z., S.W., C.K., A.S., and G.P.E. declare no conflict of interest. M.K. is the managing director of the Research Institute of Plant-Based Nutrition (IFPE) which received funding from Watson Nutrition for conducting the study. Ethical approval: The study was conducted according to the guidelines of the Declaration of Helsinki and approved by the ethics committee of the Faculty of Medicine of the University of Giessen (57/22). Consent to participate: Written consent was obtained from all subjects involved in the study.

Riboflavin supplements for blood pressure lowering in adults.

2. Cochrane Database Syst Rev. 2025 Oct 22;10(10):CD015464. doi: 10.1002/14651858.CD015464.pub2. Riboflavin supplements for blood pressure lowering in adults. Bradbury KE(1), Coffey S(2), Earle N(3), Ni Mhurchu C(1), Jull AB(4). Author information: (1)School of Population Health, University of Auckland, Auckland, New Zealand. (2)Department of Medicine, University of Otago, Dunedin, New Zealand. (3)Department of Medicine, University of Auckland, Auckland, New Zealand. (4)School of Nursing, University of Auckland, Auckland, New Zealand. Update of doi: 10.1002/14651858.CD015464. RATIONALE: Higher blood pressure is strongly related to an increased risk of cardiovascular disease. There has been interest in riboflavin (vitamin B2) as a potential intervention to lower blood pressure, particularly for those with a common genetic polymorphism in the methylene tetrahydrofolate reductase enzyme (MTHFR). OBJECTIVES: To assess the benefits and harms of riboflavin supplements compared to placebo or no additional treatment for lowering systolic and diastolic blood pressure. SEARCH METHODS: We searched the Cochrane Hypertension Specialised Register, the Cochrane Central Register of Controlled Trials (CENTRAL), Ovid MEDLINE, Ovid Embase, Clarivate Web of Science, Clarivate Food Science & Technology Abstracts and two clinical trials registries. There were no restrictions on language, publication year, or publication status. The latest search date was 24 October 2024. We also handsearched the reference lists of included studies. ELIGIBILITY CRITERIA: We included randomised controlled trials (RCTs) conducted in adults that evaluated oral riboflavin supplements compared with an inactive control (e.g. placebo or no additional treatment) and measured at least one of the critical outcomes. We did not include trials that evaluated multivitamins containing riboflavin. Participants in both the intervention and control groups were able to receive standard medical therapy for hypertension prior to and during the trial. OUTCOMES: The critical outcomes were systolic blood pressure and diastolic blood pressure. The important outcomes were antihypertensive medication, adverse events and biomarkers of riboflavin status. RISK OF BIAS: We used the Cochrane RoB 2 tool to assess the risk of bias in included studies for the following outcomes: systolic blood pressure, diastolic blood pressure, antihypertensive medication and adverse events. SYNTHESIS METHODS: We used standard Cochrane methods. For the critical outcomes, which were continuous, we calculated mean differences (MD) and 95% confidence intervals (CIs). We used a fixed-effect meta-analysis to pool data for each outcome where possible. We conducted subgroup analyses, including by MTHFR C677T genotype. INCLUDED STUDIES: We included four RCTs, which contributed 374 total participants for quantitative synthesis, although the number of participants included for each outcome varied. Most included studies for most outcomes had an overall high risk of bias. SYNTHESIS OF RESULTS: The evidence is very uncertain about the effect of riboflavin on systolic blood pressure (MD -1.94 mmHg, 95% CI -5.74 to 1.86 mmHg; P = 0.32; 3 studies, 320 participants; very low-certainty evidence) and diastolic blood pressure (MD -3.03 mmHg, 95% CI -5.97 to -0.09 mmHg; P = 0.04; 2 studies, 271 participants; very low-certainty evidence). No RCTs reported data on changes in dose or number of antihypertensive medications. One RCT (54 participants) reported data on adverse events by intervention group; there were two adverse events in the riboflavin group and one in the control group. Low-certainty evidence suggests that riboflavin may result in little to no difference in adverse events. AUTHORS' CONCLUSIONS: The evidence for the effect of oral riboflavin supplements on systolic and diastolic blood pressure is very uncertain. The evidence comes from four RCTs, three of which had an overall high risk of bias. Large, well-conducted trials are needed for high-certainty evidence of the effect of riboflavin for blood pressure lowering. FUNDING: KEB was supported by a Sir Charles Hercus Health Research Fellowship from the Health Research Council (Grant number: 19/110) and a Senior Heart Foundation fellowship from the Heart Foundation of New Zealand, supported in part by the GR Winn Trust (Grant number: 3728679). REGISTRATION: Protocol (2023): DOI: 10.1002/14651858.CD015464. Copyright © 2025 The Authors. Cochrane Database of Systematic Reviews published by John Wiley & Sons, Ltd. on behalf of The Cochrane Collaboration. DOI: 10.1002/14651858.CD015464.pub2 PMCID: PMC12541901 PMID: 41123035 [Indexed for MEDLINE] Conflict of interest statement: KEB: nothing to declare.
SC: nothing to declare.
NE: nothing to declare.
CNM: nothing to declare.
AJ: BMC Trials ‐ Other Professional Activities – Other; Health Research Council of New Zealand ‐ Grant / Contract and Other Professional Activities ‐ Data And Safety Monitoring; International Journal of Nursing Studies ‐ Employment; National Institute for Health Innovation ‐ Other Professional Activities ‐ Data And Safety Monitoring; National Institute for Health Research ‐ Other Professional Activities ‐ Data And Safety Monitoring; Smith and Nephew ‐ Travel; and University of Auckland ‐ Other Professional Activities ‐ Data And Safety Monitoring.

Antioxidant Treatment and the Chance to Conceive in Men Seeking Fertility Care: The SUMMER Randomized Clinical Trial.

3. JAMA Netw Open. 2025 Sep 2;8(9):e2532405. doi: 10.1001/jamanetworkopen.2025.32405. Antioxidant Treatment and the Chance to Conceive in Men Seeking Fertility Care: The SUMMER Randomized Clinical Trial. de Ligny WR(1)(2), de Bruin JP(2), Smits RM(1), Goovaerts IGF(3), Peeters K(3), Nap AW(1), Boxmeer JC(4), Donker RB(5), Schoonenberg M(6), Koks CAM(7), van Rumste MME(8), Visser J(9), Gielen SCJP(10), Boomsma CM(11), Smeenk JMJ(12), van Oppenraaij RHF(13), Cox T(14), Janse F(15), Muller LT(16), Brink-van der Vlugt JJ(17), Braat DDM(1), Fleischer K(6). Author information: (1)Department of Obstetrics and Gynecology, Radboud University Medical Center, Nijmegen, the Netherlands. (2)Department of Obstetrics and Gynecology, Jeroen Bosch Hospital, 's-Hertogenbosch, the Netherlands. (3)Center of Reproductive Medicine, Antwerp University Hospital, Edegem, Belgium. (4)Center of Reproductive Medicine, Voorburg Reinier de Graaf, Voorburg, the Netherlands. (5)Department of Obstetrics and Gynecology, Slingeland Hospital, Doetinchem, the Netherlands. (6)Nij Geertgen Center for Reproductive Medicine, Elsendorp, the Netherlands. (7)Department of Obstetrics and Gynecology, Máxima Medical Center, Veldhoven, the Netherlands. (8)Department of Obstetrics and Gynecology, Catharina Hospital, Eindhoven, the Netherlands. (9)Department of Obstetrics and Gynecology, Amphia Hospital, Breda, the Netherlands. (10)Department of Obstetrics and Gynecology, Franciscus Hospital, Rotterdam, the Netherlands. (11)Department of Obstetrics and Gynecology, Bravis Hospital, Roosendaal, the Netherlands. (12)Department of Obstetrics and Gynecology, Elisabeth TweeSteden Hospital, Tilburg, the Netherlands. (13)Department of Obstetrics and Gynecology, Maasstad Hospital, Rotterdam, the Netherlands. (14)Department of Obstetrics and Gynecology, Medisch Centrum Kinderwens, Leiderdorp, the Netherlands. (15)Department of Obstetrics and Gynecology, Rjinstate Hospital, Arnhem, the Netherlands. (16)Department of Obstetrics and Gynecology, Bernhoven Hospital, Uden, the Netherlands. (17)Nij Barrahûs Center for Reproductive Medicine, Wolvega, the Netherlands. IMPORTANCE: Treatments for men seeking fertility care are limited. Antioxidant supplements have been widely studied as a new treatment option, but these studies have had conflicting results. OBJECTIVE: To assess whether treatment of men seeking fertility care with an antioxidant supplement can improve semen quality and pregnancy rates compared with a placebo. DESIGN, SETTING, AND PARTICIPANTS: The SUMMER trial was a multicenter, double-blind, placebo-controlled randomized clinical trial conducted in 21 hospitals and private fertility clinics in the Netherlands. Male patients in these centers were enrolled between May 2018 and February 2024, and follow-up of the primary outcome was completed in December 2024. Eligible participants were men aged 18 to 50 years with female partners aged 18 to 43 years, who sought fertility care and were advised to undergo expectant management, treatment with intrauterine insemination, in vitro fertilization (IVF), or intracytoplasmic sperm injection (ICSI). Couples treated with ovulation induction only or IVF for bilateral tubal pathology were excluded. The men were randomly assigned to receive an antioxidant supplement or a placebo. Intention-to-treat analysis was performed for all outcomes. INTERVENTIONS: The antioxidant supplement (Impryl) was a tablet to be taken daily for 6 months. It contained betaine (200 mg), L-cystine (200 mg), niacin (16 mg), zinc (10 mg), vitamin B6 (1.4 mg), vitamin B2 (1.4 mg), folic acid (400 µg), and vitamin B12 (2.5 µg). The placebo tablet and its packaging were identical to those of the antioxidant supplement. All participating couples received standard infertility care. MAIN OUTCOMES AND MEASURES: The primary outcome was ongoing pregnancy conceived within 6 months after randomization. Secondary outcomes included semen parameters, sperm DNA fragmentation, fertilization and embryo utilization rates after IVF or ICSI, biochemical and clinical pregnancy rates, first-trimester pregnancy loss, ectopic pregnancy rate, cumulative number of pregnancies, time to pregnancy, and adverse events. RESULTS: A total of 1171 men (median [IQR] age, 34 [31-38] years; female partners' median [IQR] age, 32 [30-35] years) were included in the data analysis, of whom 591 were in the antioxidant supplement group and 580 were in the placebo group. Ongoing pregnancy rate within 6 months was not significantly different between the 2 groups (193 of 571 [33.8%] vs 208 of 555 [37.5%]; adjusted odds ratio [AOR], 0.85 [95% CI, 0.66-1.09]; P = .20). Within the window of optimal treatment effect between 4 and 6 months (considering a spermatogenesis cycle of 72 days), ongoing pregnancy rate was significantly lower in the antioxidant supplement group compared with the placebo group (69 of 446 [15.5%] vs 95 of 442 [21.5%]; AOR, 0.66 [95% CI, 0.47-0.94]; P = .02). There were no significant between-group differences for the secondary outcomes. CONCLUSIONS AND RELEVANCE: This randomized clinical trial found that ongoing pregnancy rates did not improve with the antioxidant supplement compared with a placebo. Therefore, the investigators do not support its use in men seeking fertility care. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT03337360. DOI: 10.1001/jamanetworkopen.2025.32405 PMCID: PMC12464787 PMID: 40996763 [Indexed for MEDLINE] Conflict of interest statement: Conflict of Interest Disclosures: Dr de Ligny reported receiving an unrestricted research grant and study medications from Goodlife Pharma BV during the conduct of the study and educational support from Ferring outside the submitted work. Dr de Bruin reported receiving an unrestricted research grant from Goodlife Pharma BV during the conduct of the study. Dr Smits reported receiving an unrestricted research grant from Goodlife Pharma BV during the conduct of the study. Dr van Rumste reported receiving personal fees from Ferring BV and Merck Group outside the submitted work. Dr Smeenk reported receiving grants from Merck BV, Ferring BV, and Goodlife Pharma BV outside the submitted work. Dr Fleischer reported receiving an unrestricted research grant from Goodlife Pharma BV during the conduct of the study. No other disclosures were reported.

Impact of Vitamin B1 and Vitamin B2 Supplementation on Anxiety, Stress, and Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Trial.

4. Nutrients. 2025 May 27;17(11):1821. doi: 10.3390/nu17111821. Impact of Vitamin B1 and Vitamin B2 Supplementation on Anxiety, Stress, and Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Trial. Tao Y(1), Wu M(1), Su B(1), Lin H(1), Li Q(1), Zhong T(1), Xiao Y(1), Yu X(1). Author information: (1)Faculty of Medicine, Macau University of Science and Technology, Avenida Wai Long Taipa, Macau. Background: Anxiety, stress, and sleep disturbances significantly affect overall health. Research suggests that vitamins B1 and B2 may play a role in mood regulation and neuroprotection. This study aimed to investigate the effects of vitamin B1 and B2 supplementation in alleviating anxiety and stress and improving sleep quality. Methods: This study was a parallel randomized, double-blind, placebo-controlled clinical trial. Participants (n = 43) were randomized to receive one of the following two interventions: 100 mg of vitamin B1 and 100 mg of vitamin B2 or placebo. Intervention outcomes were assessed at baseline and week four, including SAS (Self-Rating Anxiety Scale), PSS (Perceived Stress Scale), PSQI (Sleep Quality Index), ESS (Sleepiness Scale), and measurement of urinary vitamin B1 and B2 levels. Results: After four weeks, urinary vitamin B1 levels increased from 158 ± 108.9 ng to 1333.1 ± 1204.5 ng (p < 0.01), and urinary vitamin B2 levels increased from 308.0 ± 198.3 ng to 6123.2 ± 4847.2 ng in the supplement group (p < 0.01). The PSS scores decreased significantly in the supplement group from 21.5 ± 4.1 to 15.5 ± 4.5 (p < 0.05), while the placebo group showed a change from 20.3 ± 4.3 to 19.8 ± 5.5. Vitamins B1 and B2 did not have a significant effect on anxiety improvement (p > 0.05). The PSQI scores decreased in the supplement group from 8.0 ± 3.12 to 6.3 ± 2.0 (p < 0.05), while the placebo group worsened from 5.7 ± 2.7 to 7.4 ± 2.9. Meanwhile, the ESS scores in the supplement group decreased from 13.0 ± 3.4 to 9.1 ± 3.9 (p < 0.05), demonstrating a significant improvement compared to the placebo group. Conclusions: The clinical trial findings demonstrated that while vitamin B1 and B2 supplements helped reduce stress, enhance sleep, and reduce sleepiness, they had no discernible impact on reducing anxiety. Future studies should focus on the long-term effects of vitamin B1 and B2 supplements, exploring the combined effects of combined vitamin B1 and B2 medications for the treatment of stress and sleep disorders. DOI: 10.3390/nu17111821 PMCID: PMC12157762 PMID: 40507089 [Indexed for MEDLINE] Conflict of interest statement: The authors declare no conflicts of interest.

A randomized multi-arm open labelled comparative clinical trial report of Pankajakasthuri DiabetEaze powder, a novel polyherbal formulation on the nutritional management and glycemic control in type 2 diabetic and prediabetic patients.

5. Heliyon. 2025 Feb 13;11(4):e42631. doi: 10.1016/j.heliyon.2025.e42631. eCollection 2025 Feb 28. A randomized multi-arm open labelled comparative clinical trial report of Pankajakasthuri DiabetEaze powder, a novel polyherbal formulation on the nutritional management and glycemic control in type 2 diabetic and prediabetic patients. Sasidharan S(1)(2), Nair A K(3), R L(3), Nair AV(4), Sa S(5), Joseph SG(6), Chand Cp A(3), Satheesan S(7), Pratap A(3), Kumar S N(2), Paul J(8), Nair V V(9), R V(6), Nair J H(4). Author information: (1)HCEMM-SU Cardiovascular Comorbidities Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, 1089, Budapest, Hungary. (2)Department of R&D, Pankajakasthuri Herbal Research Foundation, Pankajakasthuri Ayurveda Medical College Campus, Trivandrum, India. (3)Department of Kayachikitsa, Pankajakasthuri Ayurveda Medical College & PG Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India. (4)Pankajakasthuri Herbals India Pvt. Ltd., Poovachal, Trivandrum, India. (5)Department of Rasashastra & Bhaishajya Kalpana, Pankajakasthuri Ayurveda Medical College & P.G. Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India. (6)Department of Dravyagunavijnanam, Pankajakasthuri Ayurveda Medical College & P.G. Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India. (7)Department of Shalyatantra, Pankajakasthuri Ayurveda Medical College & PG Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India. (8)Department of Statistics, Vimala College (Autonomous), Thrissur, Kerala, 680009, India. (9)Neyyar Medicity, Killy, Kattakada, Thiruvananthapuram, Kerala, India. BACKGROUND AND AIMS: Recently Diabetes Mellitus (DM) has been associated with heightened susceptibility to malnutrition, suggesting that augmenting nutritional intake stands out as a potent therapeutic strategy for addressing malnutrition in individuals with DM. The aim of this clinical investigation was to evaluate the effect of DiabetEaze powder, a polyherbal nutritional formulation developed by us for nutritional management and glycaemic control, on patients with diabetic and prediabetic conditions. METHODS: A total of 143 type II diabetic (T2D) patients who were managing their diabetic condition through modern medicine, AYUSH medicine, lifestyle modification and 68 pre-diabetic patients, aged between 40 and 65 years, were randomly assigned into six groups: control, modern, AYUSH, lifestyle, prediabetic control and prediabetic trial. The treatment groups were administered 5 g of DiabetEaze powder two times a day after food for 6 months. Microminerals, vitamins, glycaemic parameters, Quality of Life (QoL), hematology, lipid profiles, Renal Function Test (RFT) and Liver Function Test (LFL) parameters, and electrolytes were evaluated at Day 0, Day 90, and Day 180. RESULTS: Out of 211 enrolled patients, 189 individuals successfully completed the entire 180-day duration of the study, indicating a retention rate of approximately 89.6 %. In our study, we observed a statistically significant elevation in the levels of vitamin D, B2, and B6 across all treatment groups. Besides, the treatment groups displayed a notable increase in zinc and manganese levels compared to the other minerals tested. Notably, the treatment groups demonstrated distinct mineral and vitamin profiles. In terms of metabolic markers, significant reductions in Fasting Blood Sugar (FBS)/Post Prandial Blood Sugar (PPBS) were observed across the modern, AYUSH, and lifestyle groups, while the modern group also showed a marked decrease in glycated haemoglobin (HbA1c) levels. Furthermore, overall QoL among the tested groups was also statistically significant. The consistent maintenance of normal LFT and RFT parameters and electrolyte levels across trial groups throughout the study duration indicates that the supplement does not induce liver toxicity or negatively impact hepatic function. CONCLUSION: In conclusion, the nutrients present in the DiabetEaze powder contribute to the effective management of nutritional status in diabetic people and thus effectively reduce sugar spikes by regulating PPBS and HbA1c levels, which is a critical aspect of its role in diabetes management. These properties benefit in managing diabetes-related outcomes and overall quality of life. CLINICAL TRIAL REGISTRY OF INDIA UNDER REGISTRATION NO: CTRI/2021/04/032956 on 20/04/2021. © 2025 The Authors. DOI: 10.1016/j.heliyon.2025.e42631 PMCID: PMC11903805 PMID: 40083990 Conflict of interest statement: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Dr. Shan Sasidharan reports financial support was provided by Department of Pharmacology and Pharmacotherapy, Semmelweis University & Pankajakasthuri Ayurveda Medical College & PG Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India. Dr. Shan Sasidharan reports a relationship with Department of Pharmacology and Pharmacotherapy, Semmelweis University & Pankajakasthuri Ayurveda Medical College & PG Centre, Killy, Kattakada, Thiruvananthapuram, Kerala, India that includes: employment. Dr. Shan Sasidharan has patent nil pending to nil. nil If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Evaluating the Efficacy of Probiotics on Disease Progression, Quality of Life, and Nutritional Status Among Patients with Crohn's Disease: A Multicenter, Randomized, Single-Blinded Controlled Trial.

6. Nutrients. 2025 Feb 17;17(4):708. doi: 10.3390/nu17040708. Evaluating the Efficacy of Probiotics on Disease Progression, Quality of Life, and Nutritional Status Among Patients with Crohn's Disease: A Multicenter, Randomized, Single-Blinded Controlled Trial. Hoteit M(1)(2)(3), Hellani M(4)(5), Karaja M(4)(5), Zayour N(2)(3), Sadek Z(2)(6)(7), Hotayt B(8), Hallal M(4)(5). Author information: (1)Food Sciences Unit, National Council for Scientific Research of Lebanon (CNRS-L), Beirut P.O. Box 11-8281, Lebanon. (2)PHENOL Research Program, Faculty of Public Health, Section 1, Lebanese University, Beirut P.O. Box 6573, Lebanon. (3)Organized Research Unit, Zahraa University Medical Center (ZHUMC), Beirut P.O. Box 90-361, Lebanon. (4)Gastroenterology Department, Faculty of Medical Science, Lebanese University, Beirut P.O. Box 14-6573, Lebanon. (5)Gastroenterology and Hepatology Department, Zahraa University Medical Center (ZHUMC), Beirut P.O. Box 90-361, Lebanon. (6)Physiotherapy Department, Faculty of Public Health, Islamic University of Lebanon, Khaldeh P.O. Box 30014, Lebanon. (7)Physiotherapy Department, Faculty of Public Health, Lebanese University, Beirut P.O. Box 6573-1, Lebanon. (8)Badaro Endoscopic Center, Moarbes Hospital, Beirut P.O. Box 50-223, Lebanon. BACKGROUND: There is growing interest in the role of gut microbiota in the pathophysiology of inflammatory bowel diseases (IBDs), including Crohn's disease (CD). Probiotics have been proposed as a potential adjunct therapy for these conditions by altering the intestinal environment, although studies on their effectiveness have yielded mixed results. AIM: This study aims to evaluate the short-term (2 months) effects of a dietary supplement containing Lactobacilli, Bifidobacteria, and Lactococcus bacillus on disease progression, remission, quality of life, and nutritional intake in Lebanese patients with CD. METHOD: A multicenter, randomized, single-blind controlled trial was conducted in 2 medical centers in Beirut from 1 April 2024 to 1 August 2024. Recruitment, prescreening, screening, enrollment, and protocol implementation were carried out at both centers. Data were collected from 21 patients with CD, who were randomly assigned to the control group (n = 10) and the intervention group (n = 11). At baseline and after two months, participants underwent clinical assessments, WHOQOL-BREF evaluation, and 24 h dietary recalls. Follow-up visits included surveys on disease progression, quality of life, adherence, and adverse events, along with repeat body composition and anthropometric measurements. RESULTS: Probiotic supplementation over two months did not significantly alter symptoms, flares, or hospitalizations outcomes between the control and intervention groups. However, the intervention group experienced notable increases in body weight (p = 0.01), BMI (p = 0.01), body fat mass (p = 0.04), and arm muscle circumference (p = 0.01). Nutrient intake patterns differed, with the intervention group showing increased consumption of calcium, riboflavin, and folate compared to controls (p = 0.01, p = 0.04, p = 0.013, respectively). Probiotic supplementation led to significant within-group increases in dietary fiber (p = 0.01), total sugar (p = 0.02), and caffeine (p = 0.01) among the intervention participants. Adverse effects in the intervention group were mild, including nausea (18.2%) and abdominal discomfort (9.1%). QOL improved significantly in the intervention group, particularly in physical (p = 0.03), psychological (p = 0.04), and environmental domains (p = 0.003), while the control group exhibited improvements only in psychological health. CONCLUSIONS: Overall, the findings suggest that probiotics can enhance body composition, nutrient intake, and certain aspects of QOL among CD patients, despite minimal impact on disease symptoms or dietary patterns. DOI: 10.3390/nu17040708 PMCID: PMC11858769 PMID: 40005035 [Indexed for MEDLINE] Conflict of interest statement: The authors declare no conflicts of interest.

Effects of selected dietary supplements on migraine prophylaxis: A systematic review and dose-response meta-analysis of randomized controlled trials.

7. Neurol Sci. 2025 Feb;46(2):651-670. doi: 10.1007/s10072-024-07794-0. Epub 2024 Oct 15. Effects of selected dietary supplements on migraine prophylaxis: A systematic review and dose-response meta-analysis of randomized controlled trials. Talandashti MK(1), Shahinfar H(2)(3), Delgarm P(1), Jazayeri S(4). Author information: (1)Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. (2)Department of Nutrition, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran. (3)Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. (4)Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran. jazayeri.sh@iums.ac.ir. BACKGROUND: The existing evidence on the effect of dietary supplements for preventing migraines has generated conflicting results. METHODS: We assessed alterations in migraine clinical features corresponding to the intake of dietary supplements. Our main outcomes included the frequency (number of attacks), duration (in hours), the severity (intensity) and the monthly migraine days. Using a dose-response meta-analysis, we estimated the dose-dependent impact. The certainty of evidence was evaluated using the GRADE tool. RESULTS: Finally, twenty-two trials were included in the systematic review and meta-analysis. Magnesium supplementation reduced migraine attacks (mean difference (MD) = -2.51), severity (MD = -0.88), and the monthly migraine days (MD = -1.66) compared with the control group. CoQ10 decreased the frequency (MD = -1.73), severity (MD = -1.35), and duration of migraine (MD = -1.72). Riboflavin decreased attack frequency (MD = -1.34). Alpha-lipoic acid decreased attack frequency (MD = -1.24) and severity (MD = -0.38). Probiotics decreased the frequency (MD = -1.16), severity (MD = -1.07) and the monthly migraine days (MD = -3.02). Vitamin D reduced migraine frequency (MD = -1.69) and the monthly migraine days (MD = -2.41). In adults, compared with placebo, these supplements did not significantly affect other outcomes, and omega-3 supplementation did not yield a statistically significant reduction in any of these outcomes. CONCLUSION: The use of certain dietary supplements has resulted in a significant decrease in migraine prophylaxis. Further clinical trials of high quality appear to be beneficial. © 2024. Fondazione Società Italiana di Neurologia. DOI: 10.1007/s10072-024-07794-0 PMID: 39404918 [Indexed for MEDLINE] Conflict of interest statement: Declarations. Ethical approval: 1) This material is the authors’ own original work, which has not been previously published elsewhere. 2) The paper is not currently being considered for publication elsewhere. 3) The paper reflects the authors’ own research and analysis in a truthful and complete manner. 4) The authors have no conflict of interest. Conflict of interest: The authors declared no conflicts of interest.

Enhancing gut microbiota and microbial function with inulin supplementation in children with obesity.

8. Int J Obes (Lond). 2024 Dec;48(12):1696-1704. doi: 10.1038/s41366-024-01590-8. Epub 2024 Jul 20. Enhancing gut microbiota and microbial function with inulin supplementation in children with obesity. Visuthranukul C(1), Sriswasdi S(2)(3), Tepaamorndech S(4), Chamni S(5), Leelahavanichkul A(4)(6), Joyjinda Y(7)(8), Aksornkitti V(3)(9), Chomtho S(10). Author information: (1)Center of Excellence in Pediatric Nutrition, Division of Nutrition, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. chonnikant.v@chula.ac.th. (2)Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (3)Center of Excellence in Computational Molecular Biology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (4)Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (5)Center of Excellent in Natural Products and Nanoparticles (NP2), Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. (6)Center of Excellence in Inflammation and Immunology Research Unit (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (7)WHO-CC for Research and Training on Viral Zoonoses, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (8)Thai Red Cross Emerging Infection Diseases-Health Science Center, Bangkok, 10330, Thailand. (9)Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. (10)Center of Excellence in Pediatric Nutrition, Division of Nutrition, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. BACKGROUND AND OBJECTIVES: Gut dysbiosis that resulted from the alteration between host-microbe interaction might worsen obesity-induced systemic inflammation. Gut microbiota manipulation by supplementation of prebiotic inulin may reverse metabolic abnormalities and improve obesity. This study aimed to determine whether inulin supplementation improved intestinal microbiota and microbial functional pathways in children with obesity. METHODS: Children with obesity whose BMI above median + 2SDs were recruited to a randomized, double-blinded placebo-controlled study. The participants aged 7-15 years were assigned to inulin supplement extracted from Thai Jerusalem artichoke (intervention), maltodextrin (placebo), and dietary fiber advice groups. All participants received similar monthly conventional advice and follow-up for 6 months. Fecal samples were collected for gut microbiome analysis using 16S rRNA sequencing. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States was performed to infer microbial functional pathways. RESULTS: One hundred and forty-three children with available taxonomic and functional pathway abundance profiles were evaluated. A significant increase in alpha-diversity was observed in the inulin group. Inulin supplementation substantially enhanced Bifidobacterium, Blautia, Megasphaera, and several butyrate-producing bacteria, including Agathobacter, Eubacterium coprostanoligenes, and Subdoligranulum, compared to the other groups. The inulin group showed a significant difference in functional pathways of proteasome and riboflavin metabolism. These changes correlated with clinical and metabolic outcomes exclusively in the inulin group. CONCLUSIONS: Inulin supplementation significantly promoted gut bacterial diversity and improved gut microbiota dysbiosis in children with obesity. The modulation of functional pathways by inulin suggests its potential to establish beneficial interactions between the gut microbiota and host physiology. Inulin supplementation could be a strategic treatment to restore the balance of intestinal microbiota and regulate their functions in childhood obesity. © 2024. The Author(s). DOI: 10.1038/s41366-024-01590-8 PMCID: PMC11584386 PMID: 39033197 [Indexed for MEDLINE] Conflict of interest statement: Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: The research involving human subjects, human material, and human data was in accordance with the Declaration of Helsinki. The study was approved by the Institutional Review Board, Faculty of Medicine, Chulalongkorn University (IRB no. 240/60). Informed assent and consent were obtained from all participants and their parents prior to enrollment.

A Clinical Trial Shows Improvement in Skin Collagen, Hydration, Elasticity, Wrinkles, Scalp, and Hair Condition following 12-Week Oral Intake of a Supplement Containing Hydrolysed Collagen.

9. Dermatol Res Pract. 2024 Jul 10;2024:8752787. doi: 10.1155/2024/8752787. eCollection 2024. A Clinical Trial Shows Improvement in Skin Collagen, Hydration, Elasticity, Wrinkles, Scalp, and Hair Condition following 12-Week Oral Intake of a Supplement Containing Hydrolysed Collagen. Reilly DM(1), Kynaston L(1), Naseem S(1), Proudman E(1), Laceby D(1). Author information: (1)Absolute Collagen, 6 Bennetts Hill, Birmingham B2 5ST, UK. BACKGROUND: Hydrolysed collagen supplements are reported to fight the signs of aging and improve skin appearance, but more authoritative clinical evidence is needed to support efficacy. AIM: This randomised, double-blind, placebo-controlled study evaluated the efficacy of a supplement containing hydrolysed collagen and vitamin C (Absolute Collagen, AC) on biophysical parameters and visible signs of aging for skin, scalp, and hair, when taken daily or every 48 hours. METHODS: We measured dermal collagen using confocal microscopy and high-resolution ultrasound. Hydration, elasticity, wrinkles, and clinical trichoscopy were measured in parallel to expert visual grading. Efficacy measures were recorded at baseline, week 6, and week 12. RESULTS: Following 12 weeks daily use of the AC supplement, using confocal microscopy, we observed a significant 44.6% decrease in fragmentation vs. placebo (p < 0.01). We also measured a change in the ultrasound LEP (low echogenic pixel) ratio comparing upper and lower dermis (-9.24 vs. -7.83, respectively, p=0.05), suggesting collagen improvements occurred more in the upper dermal compartment. After 12 weeks vs. placebo, skin hydration was increased by 13.8% (p < 0.01), R2 elasticity index was increased by 22.7% (p<0.01), and Rz profilometry index was decreased by 19.6% (p < 0.01). Trichoscopy showed an average 11.0% improvement in scalp scaling and a 27.6% increase in the total number of hairs counted vs. placebo (p=n.s.). This was associated with a 31.9% increase in clinical grading score for hair healthy appearance (p < 0.01). CONCLUSION: The AC supplement has shown clinical benefits for skin, scalp, and hair, when used either daily or every 48 hours, over a 12-week period. Copyright © 2024 David M. Reilly et al. DOI: 10.1155/2024/8752787 PMCID: PMC11254459 PMID: 39021368 Conflict of interest statement: The authors declare that there are no conflicts of interest.

Higher Plasma Myo-Inositol in Pregnancy Associated with Reduced Postpartum Blood Loss: Secondary Analyses of the NiPPeR Trial.

10. Nutrients. 2024 Jun 27;16(13):2054. doi: 10.3390/nu16132054. Higher Plasma Myo-Inositol in Pregnancy Associated with Reduced Postpartum Blood Loss: Secondary Analyses of the NiPPeR Trial. Chang HF(1), Yong HEJ(2), Zhang H(2), Wong JT(2), Barton SJ(3), Titcombe P(3), Albert BB(4), El-Heis S(3)(5), Nield H(3), Ong J(1), Lavelle L(6), Ramos-Nieves JM(6), Godin JP(6), Silva-Zolezzi I(7), Cutfield WS(4), Godfrey KM(3)(5), Chan SY(1)(2)(8), The NiPPeR Study Group. Author information: (1)Department of Obstetrics and Gynaecology, National University Hospital, Singapore 119074, Singapore. (2)Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore 117609, Singapore. (3)MRC Lifecourse Epidemiology Centre, University of Southampton, Southampton SO16 6YD, UK. (4)Liggins Institute and a Better Start-National Science Challenge, The University of Auckland, Auckland 1023, New Zealand. (5)NIHR Southampton Biomedical Research Centre, University of Southampton & University Hospital Southampton NHS Foundation Trust, Southampton SO16 6YD, UK. (6)Nestlé Research Centre, 1000 Lausanne, Switzerland. (7)Research & Development, Nestlé Product Technology Center-Nutrition, 1800 Vevey, Switzerland. (8)Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore. We previously reported that a combined myo-inositol, probiotics, and enriched micronutrient supplement (intervention) taken preconception and in pregnancy reduced postpartum blood loss (PBL) and major postpartum hemorrhage compared with a standard micronutrient supplement (control), as secondary outcomes of the NiPPeR trial. This study aimed to identify the intervention components that may contribute to this effect. Associations of plasma concentrations of myo-inositol and vitamins B2, B6, B12, and D at preconception (before and after supplementation), early (~7-weeks), and late pregnancy (~28-weeks) with PBL were assessed by multiple linear regression, adjusting for site, ethnicity, preconception BMI, parity, and previous cesarean section. Amongst 583 women, a higher concentration of myo-inositol in early pregnancy was associated with a PBL reduction [βadj -1.26 (95%CI -2.23, -0.29) mL per µmol/L myo-inositol increase, p = 0.011]. Applying this co-efficient to the increase in mean 7-week-myo-inositol concentration of 23.4 µmol/L with the intervention equated to a PBL reduction of 29.5 mL (~8.4% of mean PBL of 350 mL among controls), accounting for 84.3% of the previously reported intervention effect of 35 mL. None of the examined vitamins were associated with PBL. Therefore, myo-inositol may be a key intervention component mediating the PBL reduction. Further work is required to determine the mechanisms involved. DOI: 10.3390/nu16132054 PMCID: PMC11242953 PMID: 38999805 [Indexed for MEDLINE] Conflict of interest statement: S.-Y.C., W.S.C., and K.M.G. are part of an academic consortium that report grants from Soci&eacute;t&eacute; Des Produits Nestl&eacute; S.A. during the conduct of the study and are co-inventors on patent filings by Nestl&eacute; S.A. relating to the NiPPeR intervention or its components. S.-Y.C. has received reimbursement and honoraria into her research funds from Nestl&eacute; Nutrition Institute and EGOI (Experts Group on Inositols) for speaking at conferences. K.M.G. has received reimbursement for speaking at conferences sponsored by companies selling nutritional products. L.L., J.M.R.-N., J.-P.G., and I.S.-Z. are employees of Soci&eacute;t&eacute; Des Produits Nestl&eacute; S.A. All other authors declare no competing interests.

The role of nutraceuticals in the management of temporomandibular disorders.

11. J Complement Integr Med. 2024 Jul 4;21(4):540-547. doi: 10.1515/jcim-2023-0394. eCollection 2024 Dec 1. The role of nutraceuticals in the management of temporomandibular disorders. Di Giacomo P(1), Forte G(2), Capogna I(1), Casagrande M(2), Di Paolo C(1). Author information: (1)Department of Oral and Maxillo-Facial Sciences, 209311 Sapienza University of Rome , Rome, Italy. (2)Department of Psychology, 209311 Sapienza University of Rome , Rome, Italy. OBJECTIVES: Temporomandibular disorders (TMDs) are usually treated with occlusal appliances and supportive treatments such as physical therapy and drugs. Supplements can be included among potential supportive therapies, with the aim of reducing the use of drugs. To evaluate the efficacy of nutraceuticals' short-term treatment in subjects with temporomandibular disorders. METHODS: The study started in January 2021 and ended in January 2022. Subjects with temporomandibular disorders and a verbal numeric scale >40 were recruited and randomly assigned to one of the following groups. If waiting to start a therapy, to the nutraceutical group or to the no treatment group, while if already undergoing splint therapy, to nutraceautical+splint group or to splint therapy group. Nutraceutical used was composed by Boswellia Serrata Casperome, Magnesium, Tryptophan and vitamins B2 and D with a posology of one tablet/day before sleep for 40 days. Presence of temporomandibular pain, headache, neck pain and sleep/emotional disorders were assessed at T0 and at T1, after 40 days. ANOVA was performed to compare treatments with nutraceuticals and their respective controls, as for the variables related to painful symptomatology. Chi-squared was conducted to assess differences in sleep/emotional disorders between groups. The statistical significance was p<0.05. RESULTS: The groups using nutraceuticals showed statistically significant improvements over controls for most of the variables analyzed. CONCLUSIONS: The use of nutraceutical seems to be a valuable support for TMD therapy in the short term either alone or combined with occlusal splint therapy. © 2024 the author(s), published by De Gruyter, Berlin/Boston. DOI: 10.1515/jcim-2023-0394 PMID: 38958698 [Indexed for MEDLINE]

Effects of Cucurbita Moschata squash (Butternut) seed paste in improving zinc and iron status in children attending Early Childhood Development centres in Limpopo province, South Africa.

12. PLoS One. 2024 Apr 18;19(4):e0300845. doi: 10.1371/journal.pone.0300845. eCollection 2024. Effects of Cucurbita Moschata squash (Butternut) seed paste in improving zinc and iron status in children attending Early Childhood Development centres in Limpopo province, South Africa. Motadi SA(1)(2), Mbhenyane XG(1), Zuma MK(1)(3), Freeland Graves JH(4). Author information: (1)Division of Human Nutrition, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa. (2)Department of Nutrition, Faculty of Health Sciences, University of Venda, Thohoyandou, South Africa. (3)Smallholder Agricultural Development, Agricultural Research Council, Pretoria, South Africa. (4)Nutritional Sciences, University of Texas at Austin, Austin, Texas, United States of America. Cucurbita moschata (Butternut squash) seeds are a rich source of nutrition containing nutrients including iron, zinc, copper, calcium, potassium, and phosphorus. The aim of this study was to determine if Cucurbita Moschata squash seed paste improves zinc and iron status, anthropometric status, and dietary intake in preschool children. A pretest-posttest control group trial using cluster randomisation was conducted over 6 months. Four preschools were randomly assigned to receive 100 g of intervention or 100 g of a placebo as the control to enhance iron and zinc status. A total of 276 preschool children were recruited from eight government registered Early Childhood Development centres in Limpopo province, South Africa. The control group consumed Cucurbita moschata flesh twice-weekly, while the intervention group consumed Cucurbita moschata seed paste twice-weekly during a six-month period. Iron (serum iron, transferrin, transferrin saturation, ferritin) and zinc (serum zinc) status and anthropometric indices such as weight, height and mid upper arm circumference for children were evaluated at baseline and the endpoint. Iron and zinc-rich food consumption was measured using a 24-hour dietary recall and food record during the study, and dietary intake was estimated using a food frequency questionnaire which was conducted at the beginning and endpoint. The intervention group significantly improved in the mean serum iron 0.23 μg/dL (95% CI: 0.11;0.33); ferritin 0.21μg/dL (95% CI: 0.13;0.39), transferrin saturation 0.33% (0.23;0.74) and zinc 0.16 μmol/dl (95% CI: 0.13;0.25) at the end of the study. In addition, the intervention group exhibited greater mean weight for age of 0.13 z-score (95% CI: 0.28; 0.34) and weight for height of 0.04 z-score (95% CI: 0.12,0.05), as well as the consumption of iron (p < 0,001), zinc (p < 0,001), and vitamin C (p < 0.001). At the end of the trial, fiber (p < 0.001), riboflavin (p = 0.001), vitamin B6 (p < 0.001), and vitamin B12 (p < 0.001) were significantly higher in the control group. Thus, the inclusion of intervention in the diet of children in an impoverished area of South Africa improved the iron and zinc status of these children. This supplement could be a cost effective and sustainable approach to improve nutrient status in rural South Africa. Trial registration: Pan African Clinical Trial Registry (PACTR202308740458863). Copyright: © 2024 Motadi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. DOI: 10.1371/journal.pone.0300845 PMCID: PMC11025940 PMID: 38635826 [Indexed for MEDLINE] Conflict of interest statement: The authors declare that they have no conflict of interest.

A nutritional supplement during preconception and pregnancy increases human milk vitamin D but not B-vitamin concentrations.

13. Clin Nutr. 2023 Dec;42(12):2443-2456. doi: 10.1016/j.clnu.2023.09.009. Epub 2023 Oct 5. A nutritional supplement during preconception and pregnancy increases human milk vitamin D but not B-vitamin concentrations. Han SM(1), Huang F(2), Derraik JGB(3), Vickers MH(1), Devaraj S(4), Redeuil K(5), Campos-Giménez E(5), Pang WW(6), Godfrey KM(7), Chan SY(8), Thakkar SK(4), Cutfield WS(9); NiPPeR Study Group. Collaborators: Albert BB, Barton SJ, Binia A, Cavanagh M, Chang HF, Chong YS, Chong MF, Conlon C, Cooper C, Costello P, Cox V, Creagh C, Depczynski M, El-Heis S, Hammond J, Harvey NC, Jagtap M, Kenealy T, Nield H, O'Sullivan JM, Satianegara G, Silva-Zolezz I, Soh SE, Tay V, Taylor R, Tham E, Titcombe P, Wall C, Wong R, Woon G, Han Z. Author information: (1)Liggins Institute, The University of Auckland, Auckland, New Zealand. (2)Nestlé Research, Société des Produits Nestlé SA, Beijing, China. (3)Liggins Institute, The University of Auckland, Auckland, New Zealand; Department of Paediatrics: Child and Youth Health, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand. (4)Nestlé Research, Société des Produits Nestlé SA, Singapore. (5)Nestlé Research, Société des Produits Nestlé SA, Lausanne, Switzerland. (6)Global Centre for Asian Women's Health, Dean's Office, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore. (7)MRC Lifecourse Epidemiology Centre, University of Southampton, Southampton, UK; NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust and University of Southampton, Southampton, UK. (8)Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore; Singapore Institute for Clinical Sciences, Agency for Science, Technology and Research, Singapore. (9)Liggins Institute, The University of Auckland, Auckland, New Zealand; A Better Start - National Science Challenge, The University of Auckland, Auckland, New Zealand. Electronic address: w.cutfield@auckland.ac.nz. BACKGROUND & AIMS: Optimal maternal vitamin status during pregnancy and lactation is essential to support maternal and infant health. For instance, vitamin D3 is involved in infant bone development, and B-vitamins are involved in various metabolic processes, including energy production. Through a double-blind randomised controlled trial, we investigated the effects of maternal supplementation from preconception throughout pregnancy until birth on human milk (HM) concentrations of vitamin D3 and B-vitamins. In addition, we aimed to characterise longitudinal changes in milk concentrations of these vitamins. METHODS: Both control and intervention supplements contained calcium, iodine, iron, β-carotene, and folic acid, while the intervention also contained zinc, vitamins B2, B6, B12, and D3, probiotics, and myo-inositol. HM samples were collected across 4 time points from 1 week to 3 months post-delivery from 158 mothers in Singapore, and 7 time points from 1 week to 12 months from 180 mothers in New Zealand. HM vitamin D was quantified using supercritical fluid chromatography and B-vitamins with mass spectrometry. Potential intervention effects on HM vitamins D3, B2, B6, and B9, as well as other B-vitamin (B1 and B3) concentrations were assessed using linear mixed models with a repeated measures design. RESULTS: Over the first 3 months of lactation, HM 25-hydroxyvitamin D3 concentrations were 20% (95% CI 8%, 33%, P = 0.001) higher in the intervention group, with more marked effects in New Zealand. There were no observed intervention effects on HM concentrations of vitamins B1, B2, B3, B6, and B9. In New Zealand mothers, longitudinally, vitamin D3 concentrations gradually increased from early lactation up to 12 months, while vitamins B1 and B2 peaked at 6 weeks, B3 at 3 weeks, and B6 and B9 at 3 months. CONCLUSIONS: Maternal supplementation during preconception and pregnancy increased HM vitamin D, but not B-vitamin concentrations in lactation. Further studies are required to examine the discrete benefits of vitamin D supplementation starting preconception vs during pregnancy, and to further characterise the effects of supplementation on later offspring health outcomes. CLINICAL TRIAL REGISTRATION: Registered at ClinicalTrials.gov on the 16 July 2015 (identifier NCT02509988); Universal Trial Number U1111-1171-8056. This study was academic-led by the EpiGen Global Research Consortium. Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved. DOI: 10.1016/j.clnu.2023.09.009 PMID: 38411017 [Indexed for MEDLINE]

From A to E: Uniting vitamins against stroke risk-A systematic review and network meta-analysis.

14. Eur J Clin Invest. 2024 Jun;54(6):e14165. doi: 10.1111/eci.14165. Epub 2024 Jan 30. From A to E: Uniting vitamins against stroke risk-A systematic review and network meta-analysis. Tripathi S(1), Nath M(1), Misra S(2), Kumar P(3). Author information: (1)Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. (2)Department of Neurology, Yale University, New Haven, Connecticut, USA. (3)Clinical Research Unit, All India Institute of Medical Sciences, New Delhi, India. BACKGROUND AND AIM: Stroke represents a significant public health challenge, necessitating the exploration of preventive measures. This network meta-analysis aimed to assess the efficacy of different vitamin treatments compared to a placebo in preventing stroke. METHODS: A systematic electronic search in databases including PubMed, EmBASE, Web of Science, clinicaltrials.gov, and Google Scholar until 31 May 2023 was conducted, to identify published studies investigating the association between vitamin intake and the risk of stroke. Pooled risk ratio (RR) with 95% confidence intervals (CIs) was calculated using a frequentist network meta-analysis. Furthermore, we ranked vitamins based on p-scores, facilitating a comparative assessment of their effectiveness in preventing stroke. RESULTS: A total of 56 studies, including 17 randomized controlled trials (RCTs) and 39 cohort studies were analyzed. Direct estimates obtained from network meta-analysis, we found that vitamin A (RR: .81 [.72-.91]), vitamin B-complex (RR: .85 [.74-.97]), vitamin B6 (RR: 79 [.68-.92]), folate (RR: .86 [.75-.97]), vitamin C (RR: .77 [.70-.85]) and vitamin D (RR: .73 [.64-.83]) were significantly associated with a decreased stroke risk. However, no significant association was observed for vitamin B2, vitamin B12, and vitamin E. Subsequent to network meta-analysis, vitamins were ranked in decreasing order of their efficacy in stroke prevention based on p-score, with vitamin D (p-score = .91), vitamin C (p-score = .79), vitamin B6 (p-score = .70), vitamin A (p-score = .65), vitamin B-complex (p-score = .53), folate (p-score = .49), vitamin B2 (p-score = .39), vitamin E (p-score = .28), vitamin B12 (.13) and placebo (.10). CONCLUSION: Our study has established noteworthy connections between vitamin A, vitamin B-complex, vitamin B6, folate, vitamin C, and vitamin D in the realm of stroke prevention. These findings add substantial weight to the accumulating evidence supporting the potential advantages of vitamin interventions in mitigating the risk of stroke. However, to solidify and validate these observations, additional research is imperative. Well-designed clinical trials or cohort studies are needed to further explore these associations and formulate clear guidelines for incorporating vitamin supplementation into effective stroke prevention strategies. © 2024 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd. DOI: 10.1111/eci.14165 PMID: 38291560 [Indexed for MEDLINE]

Effects of a low-protein nutritional formula with dietary counseling in older adults with chronic kidney disease stages 3-5: a randomized controlled trial.

15. BMC Nephrol. 2023 Dec 14;24(1):372. doi: 10.1186/s12882-023-03423-8. Effects of a low-protein nutritional formula with dietary counseling in older adults with chronic kidney disease stages 3-5: a randomized controlled trial. Yang WC(1), Hsieh HM(1), Chen JP(2), Liu LC(1), Chen CH(3)(4)(5)(6). Author information: (1)Department of Food and Nutrition, Taichung Veterans General Hospital, Taichung, Taiwan. (2)Biostatistics Group, Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. (3)Division of Nephrology, Department of Internal Medicine, Taichung Veterans General Hospital, No. 1650, Sect. 4, Taiwan Boulevard, Taichung, Taiwan. cschen920@yahoo.com. (4)Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University School of Medicine, Taichung, Taiwan. cschen920@yahoo.com. (5)Department of Life Science, Tunghai University, Taichung, Taiwan. cschen920@yahoo.com. (6)Program in Tissue Engineering and Regenerative Medicine, College of Medicine, National Chung-Hsing University, Taichung, Taiwan. cschen920@yahoo.com. BACKGROUND: Although combining a low-protein diet (LPD) with oral nutritional supplements increases treatment adherence and nutritional status in patients with chronic kidney disease (CKD), the effect of this combination approach in older adults remains unclear. This study examined the impact of a 6% low-protein formula (6% LPF) with diet counseling in older adults with stage 3-5 CKD. METHODS: In this three-month randomized controlled study, 66 patients (eGFR < 60 mL/min/1.73 m2, non-dialysis, over 65 years of age) were randomly assigned to an intervention group (LPD plus a 6% LPF) or control group (LPD alone). The 6% LPF comprised 400 kcal, 6 g of protein, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and various micronutrients. All data were collected at baseline and after three months, including physical performance based on hand grip strength (HGS) and gait speed, nutritional status using Mini Nutritional Assessment-Short Form (MNA-SF) scores, body composition through bioelectrical impedance analysis, and dietary intake from 24-h dietary records. RESULTS: This study incorporated 47 participants (median age, 73; median eGFR, 36 ml/min/1.73 m2; intervention group: 24; control group: 23). The intervention group exhibited significant differences in HGS and gait speed, and micronutrient analysis revealed significantly higher monounsaturated fatty acids (MUFA), EPA, DHA, calcium, iron, zinc, copper, thiamine, riboflavin, niacin, B6, B12, and folic acid intake than the control group. MNA-SF scores, macronutrient intake, and body composition did not differ significantly between the two groups. CONCLUSIONS: Compared to LPD counseling alone, an LPD prescription with 6% LPF in older adults with CKD stages 3-5 helped relieve physical deterioration and increased micronutrient intake after three months. TRIAL REGISTRATION: ClinicalTrials.gov NCT05318014 (retrospectively registered on 08/04/2022). © 2023. The Author(s). DOI: 10.1186/s12882-023-03423-8 PMCID: PMC10720150 PMID: 38097963 [Indexed for MEDLINE] Conflict of interest statement: The authors declare no competing interests.

Intrinsic multi-component fluorescence fingerprint of bovine milk-derived exosomes for label-free biosensing.

1. Spectrochim Acta A Mol Biomol Spectrosc. 2026 Jun 14;363(Pt 1):128259. doi: 10.1016/j.saa.2026.128259. Online ahead of print. Intrinsic multi-component fluorescence fingerprint of bovine milk-derived exosomes for label-free biosensing. Aray A(1), Morshedi A(2), Ramezani R(3), Shokoufi N(4). Author information: (1)Department of Atomic and Molecular Physics, Faculty of Physics, Alzahra University, Tehran, Iran. Electronic address: a.aray@alzahra.ac.ir. (2)Teifsanje Co., Technology Incubator Center, CCERCI, Tehran, Iran. (3)Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. (4)Department of Analytical Chemistry, Analytical Instrumentation & Spectroscopy Laboratory at Chemistry and Chemical Engineering Research Center of Iran (CCERCI), Tehran, Iran. Electronic address: n.shokoufi@ccerci.ac.ir. Bovine milk-derived exosomes are biocompatible nanovesicles with significant potential for drug delivery, yet reliable methods for their label-free identification remain limited. This study employs fluorescence spectroscopy to characterize the intrinsic optical fingerprint of BMEs. Initially, the Excitation-Emission Matrix served as an exploratory tool to map the spectral landscape. Second-derivative analysis was then applied to resolve intrinsic fluorophore hotspots obscured by background interference, followed by spectral deconvolution to separate overlapping components. This analysis revealed a distinct multi-component fluo

Treatment outcomes in new daily persistent headache in children and adolescents.

2. Cephalalgia. 2026 Jun;46(6):3331024261426562. doi: 10.1177/03331024261426562. Epub 2026 Jun 19. Treatment outcomes in new daily persistent headache in children and adolescents. Abuhalaweh N(1)(2), Gelfand AA(3), Evans M(2), Marquez de Prado B(2), Patterson Gentile C(1)(2), Raj N(2), Hershey AD(4)(5), Szperka CL(1)(2). Author information: (1)Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. (2)Pediatric Headache Program, Children's Hospital of Philadelphia, Philadelphia, PA, USA. (3)Department of Neurology, Child & Adolescent Headache Program, University of California San Francisco, San Francisco, CA, USA. (4)Division of Neurology, Cincinnatti Children's Hospital & Medical Center, San Cincinnatti, OH, USA. (5)Department of Pediatrics, University of Cincinnati School of Medicine, San Cincinnatti, OH, USA. BackgroundNew daily persistent headache (NDPH) is a primary headache disorder that often presents in adolescence. Presently, there is no effective treatment for NDPH and a paucity of clinical trials exploring therapeutic options. In this study, we explored the relative benefit of currently used treatments to help inform future trials and clinical decision-making.MethodsIn this retrospective chart review study, patients aged 5-17 years with abrupt onset continuous headache and headache duration of at least one month (constituting NDPH or probable NDPH) were identified based on responses to a Headache Questionnaire in child neu

Effects of different protein sources on rumen fermentation-microbial characteristics, nutrient utilization, and fecal and urinary metabolites in periparturient breeding cows.

3. Front Microbiol. 2026 Jun 3;17:1834901. doi: 10.3389/fmicb.2026.1834901. eCollection 2026. Effects of different protein sources on rumen fermentation-microbial characteristics, nutrient utilization, and fecal and urinary metabolites in periparturient breeding cows. Ren Y(1), Ma C(1)(2), Zhang J(1)(3), Ma L(1), Qin Y(1), Zhang Q(1). Author information: (1)College of Animal Science and Technology, Ningxia University, Yinchuan, China. (2)Ningxia Hui Autonomous Region Veterinary Drug and Feed Inspection Institute, Yinchuan, China. (3)Yongning County Animal Husbandry and Aquaculture Technology Extension Service Center Yongning, Yinchuan, China. INTRODUCTION: As a core nutrient regulating ruminant metabolic networks, immune homeostasis, and rumen microbial community structure, the source and supply of protein influence the adaptive capacity of breeding cows to metabolic stress during physiological transitions in the peripartum period. METHODS: Sixty healthy, periparturient cows with similar body weights (573.14 ± 76.67 kg) and gestation lengths (228.77 ± 37.86 d) were selected and randomly divided into four groups (n = 15), each group received an isocaloric, isonitrogenous diet supplemented with soybean meal (Control Group, CG), rapeseed meal (Test Group 1, TG1), linseed cake (Test Group 2, TG2), or sunflower cake (Test Group 3, TG3) as the primary protein source. Rumen fermentation parameters, microbial composition and community structure, serum indicators,

Effect of pH on the formation mechanism of riboflavin mediated photocrosslinking hydrogel of soybean protein nanofibrils.

4. Int J Biol Macromol. 2026 Jun 18:153111. doi: 10.1016/j.ijbiomac.2026.153111. Online ahead of print. Effect of pH on the formation mechanism of riboflavin mediated photocrosslinking hydrogel of soybean protein nanofibrils. Zhang X(1), Zhao H(1), Lu Y(1), Simonenko SV(2), Simonenko ES(2), Tong LT(3), Li Y(4). Author information: (1)College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China. (2)The Research Institute of Baby Food, 1435500 Moscow Region, Istra, Moscow, St. 48, Russia. (3)Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, 100193, China. Electronic address: tonglitao@caas.cn. (4)College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China; College of Food Science and Engineering, Jilin University, Changchun, 130062, China; Chongnqing Research Institute, Harbin Institute of Technology, Chongqing, 401135, China. Electronic address: yangli@neau.edu.cn. Riboflavin (RF) mediated photocrosslinking provides a green and controllable approach for soy protein nanofibril (SPF) hydrogel, but the pH-dependent reaction efficiency and structural stability remain key challenges to achieve reliable and sustainable applications. In this study, RF was used as a photosensitizer to induce the crosslinking of SPF under 365 nm UV irradiation, and the gelation mechanism of the photocros

Initial exploration of the health effects on Qinghai-Tibetan Plateau yaks following short-term exposure to polystyrene microplastics: Analysis of rumen microbiota, host metabolism, antioxidant function and inflammatory responses.

5. J Hazard Mater. 2026 Jun 13;514:142707. doi: 10.1016/j.jhazmat.2026.142707. Online ahead of print. Initial exploration of the health effects on Qinghai-Tibetan Plateau yaks following short-term exposure to polystyrene microplastics: Analysis of rumen microbiota, host metabolism, antioxidant function and inflammatory responses. Zhang P(1), Zhu Y(2), Wang Z(3), Yu P(4), Xue B(5), Wang L(6), Hu R(7), Zou H(8), Jiang Y(9), Xiao J(10), Tan C(11), Wu F(12), Peng Q(13). Author information: (1)Animal Nutrition Institute, Key laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: zhangph48468@163.com. (2)Institute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agriculture and Animal Husbandry Science, Lhasa 850009, China. Electronic address: 1269919750@qq.com. (3)Animal Nutrition Institute, Key laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: wangzs67@163.com. (4)Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, SK S7N 5A8, Canada. Electronic address: Peiqiang.yu@usask.ca. (5)Animal Nutrition Institute, Key laboratory of Animal Disease-Resistance Nutrition, Ministry of Edu

Integrating network pharmacology and MALDI-MSI spatial metabolomics revealing ellagic acid as the key anti-pharyngitis agent in Fructus Chebulae and its key metabolic pathways in pharyngeal tissues.

6. Food Funct. 2026 Jun 18. doi: 10.1039/d5fo05286f. Online ahead of print. Integrating network pharmacology and MALDI-MSI spatial metabolomics revealing ellagic acid as the key anti-pharyngitis agent in Fructus Chebulae and its key metabolic pathways in pharyngeal tissues. Kong Y(1), Wang C(1), Zhang Y(1), Luo X(2), Hu F(1), Chen J(1), Chen X(1). Author information: (1)School of Pharmacy, Lanzhou University, No. 222, Tianshui Road, Lanzhou 730000, Gansu Province, P.R. China. hufd@lzu.edu.cn. (2)Northwest Minzu University, Lanzhou 730000, P.R. China. Current pharyngitis treatment relies heavily on antibiotics and glucocorticoids, leading to increasing bacterial resistance and significant adverse drug reactions, while the traditional efficacy of Fructus Chebulae is to reduce swelling and relieve sore throat, yet its key components and mechanism against pharyngitis need systematic research. Therefore, this study aimed to integrate MALDI-MSI spatial metabolomics and network pharmacology to systematically analyze Fructus Chebulae's therapeutic mechanism against acute pharyngitis, identifying ellagic acid as the core component, along with key targets and spatial metabolic pathways. Using a 'disease-component-metabolic pathways' strategy, network pharmacology was combined with in vivo validation in an acute pharyngitis animal model induced by 5% ammonia oropharyngeal spray, followed by spatial metabolomics analysis. The results showed that network pharmacology id

Toward clinically relevant automated corneal biomanufacturing with human-derived FBS alternatives.

7. Sci Rep. 2026 Jun 17;16(1):18874. doi: 10.1038/s41598-026-58401-5. Toward clinically relevant automated corneal biomanufacturing with human-derived FBS alternatives. Taoum A(1), Oster JS(2), Thaden O(2), Frank A(2), Wang M(2), Wisbar M(2), Fuest M(3), Dehli F(2), Duarte Campos DF(4). Author information: (1)Bioprinting & Tissue Engineering Group, Center for Molecular Biology of Heidelberg University, Heidelberg, Germany. a.taoum@zmbh.uni-heidelberg.de. (2)Bioprinting & Tissue Engineering Group, Center for Molecular Biology of Heidelberg University, Heidelberg, Germany. (3)Department of Ophthalmology, RWTH Aachen University, Aachen, Germany. (4)Bioprinting & Tissue Engineering Group, Center for Molecular Biology of Heidelberg University, Heidelberg, Germany. dcampos@uni-heidelberg.de. The replacement of animal-derived components from cell culture protocols is essential for the development of human-compatible and clinically translatable systems. Fetal bovine serum (FBS) is still widely used for cell expansion, although its xenogeneic origin and batch variability limit regulatory compliance. In this study, human platelet lysate (hPL) and human serum (HS) were assessed as low-serum (2%) alternatives to FBS during the subculture phase of bone marrow-derived mesenchymal stromal cells (BM-MSC) prior to keratocyte differentiation for corneal tissue engineering. BM-MSC were gradually adapted over four days to 2% FBS, hPL, or HS, maintained for seven days, and then

Valorization of fruit by-products: Vitamin B12 fortification in fruit pomace using a co-culture of propionic and lactic acid bacteria.

8. Int J Food Microbiol. 2026 Jun 15;459:111910. doi: 10.1016/j.ijfoodmicro.2026.111910. Online ahead of print. Valorization of fruit by-products: Vitamin B12 fortification in fruit pomace using a co-culture of propionic and lactic acid bacteria. Piwowarek K(1), Makowska Z(2), Kowalczyk M(3), Kern-Zdanowicz I(4), Ruzik L(5), Synowiec A(6). Author information: (1)Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska St. 159c, 02-776, Warsaw, Poland. Electronic address: kamil_piwowarek@sggw.edu.pl. (2)Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska St. 159c, 02-776, Warsaw, Poland. Electronic address: z.makowska60@gmail.com. (3)Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego St. 5a, 02-106, Warsaw, Poland. Electronic address: mk@ibb.waw.pl. (4)Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego St. 5a, 02-106, Warsaw, Poland. Electronic address: iza@ibb.waw.pl. (5)Chair of Analytical Chemistry, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664, Warsaw, Poland. Electronic address: lena.ruzik@pw.edu.pl. (6)Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska St. 159c, 02-776, Warsaw, Poland. Electronic address: alicja_synowiec@sggw.edu.pl.

Genomic evolution of Francisella: metabolic innovation, endosymbiotic transitions to ticks, and biogeographic history.

9. Genome Biol Evol. 2026 Jun 16:evag135. doi: 10.1093/gbe/evag135. Online ahead of print. Genomic evolution of Francisella: metabolic innovation, endosymbiotic transitions to ticks, and biogeographic history. Echeverry-Pérez JS(1), Castelli M(2), Muñoz-Leal S(3), Nava S(4), Sassera D(2)(5), Sánchez-Vialas A(1), Olmeda AS(6), Valcárcel F(7), Uribe JE(1)(8)(9). Author information: (1)Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (MNCN-CSIC), Madrid, Spain. (2)Department of Biology and Biotechnology, University of Pavia, Italy. (3)Departamento de Ciencia Animal, Facultad de Ciencias Veterinarias, Universidad de Concepción, Chillán, Chile. (4)Estación Experimental Agropecuaria Rafaela (EEA Rafaela), Instituto Nacional de Tecnología Agropecuaria, Santa Fe, Argentina. (5)IRCCS Fondazione Policlinico San Matteo, Pavia,  Italy. (6)Departamento de Sanidad Animal, Universidad Complutense de Madrid, Spain. (7)Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, Spain. (8)Department of Bioscience, University of Milano, Italy. (9)Department of Invertebrate Zoology, MRC 163, National Museum of Natural History, Smithsonian Institution, 1000 Madison Drive NW, Washington, 20013-7012 DC, USA. Ticks (Ixodida) are the second most important vectors of infectious diseases in vertebrates, after mosquitoes. Beyond vector roles, they maintain mutualistic associations

Association between dietary thiamin, riboflavin and niacin intake and hypertension and increased pulse pressure: A cross-sectional study of the National Health and Nutrition Examination Survey 1999-2023.

10. Medicine (Baltimore). 2026 Jun 12;105(24):e49149. doi: 10.1097/MD.0000000000049149. Association between dietary thiamin, riboflavin and niacin intake and hypertension and increased pulse pressure: A cross-sectional study of the National Health and Nutrition Examination Survey 1999-2023. Ma S(1), Jin Q(2), Yu K(2), Dong H(1), Lu Y(1), Chang L(2). Author information: (1)Department of Cardiology, Changchun University of Traditional Chinese Medicine, Changchun, China. (2)Cardiology Department, Changchun University of Traditional Chinese Medicine Affiliated Hospital, Changchun, China. This cross-sectional study aimed to examine the associations between total intake of 3 B vitamins (thiamin, riboflavin, niacin) and hypertension and increased pulse pressure in U.S. adults, and to explore potential nonlinear relationships.Data from the U.S. National Health and Nutrition Examination Survey (NHANES) 1999-2023 were analyzed. A total of 17,484 participants aged ≥ 20 years were included. Total vitamin intake (diet plus supplements) was categorized into quartiles. Hypertension was defined as SBP ≥ 140 mm Hg or DBP ≥ 90 mm Hg; increased pulse pressure as PP ≥ 60 mm Hg. Multivariable logistic regression and restricted cubic spline (RCS) models were used, adjusting for demographic, socioeconomic, and clinical factors.In fully adjusted models, the highest quartile of total thiamin (OR 1.17, 95% CI 1.04-1.31), riboflavin (OR 1.23, 95% CI 1.10-1.39), and niacin (OR 1.16,

Defining Rational Photoelectron Routing for Targeted Intracellular Energy Transfer.

11. Angew Chem Int Ed Engl. 2026 Jun 16:e2408585. doi: 10.1002/anie.2408585. Online ahead of print. Defining Rational Photoelectron Routing for Targeted Intracellular Energy Transfer. Wang H(1)(2), Li J(2), Feng Y(1), Wang L(2), He D(2), Zeng C(2), Cai Z(1), Xiao K(2), Wang B(2). Author information: (1)Technology Innovation Center For Marine Ecology and Human Factor Assessment of Natural Resources Ministry, Tsinghua Shenzhen International Graduate School, Shenzhen, Guangdong Province, P. R. China. (2)State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Key Laboratory of Materials Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, P. R. China. Microbial artificial photosynthesis offers a promising strategy for light-driven biomanufacturing, yet its efficiency remains limited by the non-selective conversion of photogenerated electrons into metabolically usable reducing power, causing energy dissipation and weak coupling between light capture and metabolic reactions. Here, we report a rational strategy using riboflavin (RF), a membrane-permeable and biocompatible flavin photosensitizer, to selectively channel photonic energy into intracellular NADPH regeneration. Quantum chemical calculations and spectroscopic analyses reveal that light-excited RF exhibits a specific binding affinity and favorable electron transfer trend toward NADP+. In vivo, RF activatio

Central Nervous System T-cell immune architecture, and not HIV burden, tracks with cognition under long-term viral suppression.

12. PLoS Pathog. 2026 Jun 15;22(6):e1014351. doi: 10.1371/journal.ppat.1014351. Online ahead of print. Central Nervous System T-cell immune architecture, and not HIV burden, tracks with cognition under long-term viral suppression. Trunfio M(1)(2), Caballero G(1), Gomez-Moreno V(1), Mallal SA(3)(4), Wanjalla CN(5)(6), Jones A(4), Beeri K(4), Wells A(1), LaMere S(1), Gouaux B(7), Franklin DR(7), Corley M(8), Ellis RJ(7), Moore DJ(7), Letendre SL(1)(7), Smith D(1), Chaillon A(1), Gianella S(1). Author information: (1)Division of Infectious Diseases and Global Health, Department of Medicine, University of California San Diego, La Jolla, California, United States of America. (2)Department of Clinical and Biological Sciences, University of Turin, Torino, Italy. (3)Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America. (4)Vanderbilt Technologies for Advanced Genomics, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America. (5)Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, Tennessee, United States of America. (6)Veterans Affairs Tennessee Valley Healthcare System, Nashville, Tennessee, United States of America. (7)Department of Psychiatry, University of California San Diego, San Diego, California, United States of America. (8)Division of Geriatrics and Palliative Care, Department of Medicine, University of California San Diego, La Jolla, California,

Theranostic-Guided Corneal UV Therapy for Progressive Keratoconus: First Real-World Evidence One-Year Clinical Outcomes.

13. Cornea. 2026 Jun 11. doi: 10.1097/ICO.0000000000004187. Online ahead of print. Theranostic-Guided Corneal UV Therapy for Progressive Keratoconus: First Real-World Evidence One-Year Clinical Outcomes. Fruschelli M(1), Mencucci R(2), Chiavetta E(1), Carnicci A(1), Alunni Fegatelli D(3), Vestri A(4), Lombardo G(5)(6), Lombardo M(6)(7). Author information: (1)Ophthalmology Unit, Dept. Medical-Surgical Sciences and Neuroscience, Santa Maria alle Scotte University Hospital, Siena, Italy. (2)Ophthalmology Unit, Careggi Hospital, University of Firenze, Firenze, Italy. (3)Department of Life Sciences, Health and Health Professions, Link Campus University, Rome, Italy. (4)Department of Public Health and Infectious Diseases, University of Rome "La Sapienza", Rome, Italy. (5)CNR-IPCF, Istituto per i Processi Chimico-Fisici, Messina, Italy. (6)Vision Engineering Italy srl, Rome, Italy; and. (7)Studio Italiano di Oftalmologia, Rome, Italy. PURPOSE: To assess the efficacy of theranostic-guided corneal UV therapy in progressive keratoconus and the association between theranostic imaging biomarkers and 1-year outcomes. METHODS: Seventy-three consecutive patients (73 eyes; mean age 22 ± 6 years) with progressive keratoconus underwent epithelial removal followed by theranostic-guided corneal UV therapy using 0.22% riboflavin ophthalmic solution. Eyes received either 10- or 15-minute preset riboflavin application protocol, followed by UV irradiation at 10 mW/cm2 for 9 minutes.

Virgin Coconut Oil Attenuates Diabetic Kidney Disease via Gut Microbiota-Metabolism-Inflammation Axis Modulation in Type 2 Diabetic Mice.

14. Food Sci Nutr. 2026 Jun 11;14(6):e71973. doi: 10.1002/fsn3.71973. eCollection 2026 Jun. Virgin Coconut Oil Attenuates Diabetic Kidney Disease via Gut Microbiota-Metabolism-Inflammation Axis Modulation in Type 2 Diabetic Mice. Shao K(1), Cao Y(1), Gao R(2), Dong X(3), Liu X(1), Xu H(3), Xie Y(3), Xu L(1), He J(4), Chen M(5), Zhang L(3), Ali A(3)(6), Wu L(3), Wang P(2). Author information: (1)Department of Laboratory Medicine The Yancheng Clinical College of Xuzhou Medical University, The First People's Hospital of Yancheng Yancheng China. (2)Department of Laboratory Medicine Taizhou Second People's Hospital Taizhou China. (3)Department of Laboratory Medicine, School of Medicine Jiangsu University Zhenjiang China. (4)Health Testing Center Zhenjiang Center for Disease Control and Prevention Zhenjiang China. (5)Public Experiment and Service Center Jiangsu University Zhenjiang China. (6)Department of Pulmonary Medicine Abbassia Chest Hospital, EMOH Cairo Egypt. Diabetic kidney disease (DKD) poses a significant global health challenge, necessitating novel therapeutic interventions. Virgin coconut oil (CO), rich in medium-chain fatty acids (MCFAs), has emerging metabolic benefits, but its renoprotective potential and mechanistic basis remain unexplored. This study aimed to investigate the therapeutic effects of CO on DKD and elucidate its underlying molecular mechanisms through a multimodal approach. Network pharmacology and molecular docking were employed to pr

A functional betalain-producing dioxygenase in Neolecta irregularis reveals an expanded evolutionary origin of the pathway.

15. IMA Fungus. 2026 Jun 5;17:e184549. doi: 10.3897/imafungus.17.184549. eCollection 2026. A functional betalain-producing dioxygenase in Neolecta irregularis reveals an expanded evolutionary origin of the pathway. Martínez-Rodríguez P(1), Guerrero-Rubio MA(1)(2), Henarejos-Escudero P(1)(3), Hernández-García S(1), Gandía-Herrero F(1). Author information: (1)Departamento de Bioquímica y Biología Molecular A, Unidad Docente de Biología, Facultad de Veterinaria, Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain Regional Campus of International Excellence "Campus Mare Nostrum", Universidad de Murcia Murcia Spain https://ror.org/03p3aeb86. (2)Universidad Europea de Valencia, Faculty of Health Sciences, Universidad Europea de Valencia, Paseo de la Alameda 7, 46010, Valencia, Spain Faculty of Health Sciences, Universidad Europea de Valencia Valencia Spain https://ror.org/05m35m789. (3)Current Address: Molecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, Universidad Católica de Murcia (UCAM), Campus de los Jerónimos 135, 30107 Guadalupe, Spain Health Sciences Department, Universidad Católica de Murcia (UCAM) Guadalupe Spain. Betalains are nitrogen-containing pigments with important biological, ecological, and biotechnological roles, yet their biosynthesis has been described almost exclusively in plants of the order Caryophyllales and in a few fung

Dual-Mode Fluorescence Turn-On Bentazone Determination and Ratiometric/Colorimetric Vitamin B2 Quantification Based on an AIE-Active Zn MOF Sensor.

1. Inorg Chem. 2026 Aug 17;65(32):18862-18873. doi: 10.1021/acs.inorgchem.6c02688. Dual-Mode Fluorescence Turn-On Bentazone Determination and Ratiometric/Colorimetric Vitamin B2 Quantification Based on an AIE-Active Zn MOF Sensor. Li YH(1)(2)(3), Li L(1), You SY(2), Zhang L(2), Zeng Q(2), Chen Z(3), Zou JY(2). Author information: (1)Institute of Medicine and Health, Jiangxi Academy of Sciences , Nanchang330096, P. R. China. (2)Institute of Materials and Intelligent Manufacturing, Jiangxi Academy of Sciences , Nanchang330096, P. R. China. (3)Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang330013, P. R. China. Monitoring bentazone (BEN) residues in agricultural products and quantifying vitamin B2 (VB2) in fortified foods are essential for ensuring food safety and regulatory compliance. Herein, an AIE-active zinc-based metal-organic framework, {[ZnNa(HTCPP)]·3H2O·DMF}n (ZnTCPP, where H4TCPP = 2,3,5,6-(4-carboxyl-tetraphenyl)pyrazine) was synthesized and explored as a dual-mode fluorescence sensor for turn-on BEN determination and ratiometric/colorimetric VB2 quantification. Sensing mechanisms involve the collaborative contribution of competitive absorption, photoinduced electron transfer (PET), and host-guest interactions for BEN, and Förster resonance energy transfer (FRET) for VB2. ZnTCPP exhibited remarkable fluorescence enhancement toward BEN with a constant of 5.14 × 104 M-1 and a detection limit of

Development of Vitamin-fortified rice analogs with a focus on extrusion and cooking stability and retention.

2. J Food Sci Technol. 2026 Sep;63(9):1682-1690. doi: 10.1007/s13197-025-06337-5. Epub 2025 May 26. Development of Vitamin-fortified rice analogs with a focus on extrusion and cooking stability and retention. Ganachari A(1), Nidoni U(2), Hiregoudar S(2), Ganachari S(3), K T R(2), Naik N(2), S V(4), Mathad PF(2). Author information: (1)Zonal Agricultural Research Station, Kalaburagi-03, (UAS Raichur), Raichur, Karnataka India. (2)Department of Processing & Food Engg., College of Agril. Engg., UAS Raichur, Raichur, Karnataka India. (3)School of Advanced Sciences, KLE Technological University, B. V. Bhoomaraddi Campus, Vidyanagar, Hubballi, Karnataka India. (4)Home Science, AEEC Lingasugur, UAS Raichur, Raichur, Karnataka India. Rice, a global staple, often lacks essential nutrients due to milling, leading to micronutrient deficiencies or "hidden hunger." This study aimed to optimize the fortification of rice analogues with essential vitamins, focusing on nutrient retention during cooking. Using cold extrusion technology, rice analogues were produced from a mixture of rice flour, sodium alginate (1%), water (30%), and a nutrient ready mix (NRM) in varying proportions: low-dose (100%), intermediate-dose (125%), and High-dose (150%) of recommended levels. The fortification level was statistically optimized using Design Expert software, with high-dose fortification (150% NRM) yielding the highest cooking retention, achieving a desirability value of 0.961. Post-co

Bioaccessibility of selected vitamins and minerals in dairy products and plant-based alternatives using the INFOGEST 2.0 in vitro digestion model.

3. Food Chem. 2026 Jul 24;526:150528. doi: 10.1016/j.foodchem.2026.150528. Online ahead of print. Bioaccessibility of selected vitamins and minerals in dairy products and plant-based alternatives using the INFOGEST 2.0 in vitro digestion model. Kasimir M(1), Jensen MB(1), Amlund H(2), Sloth JJ(2), Lillevang SK(3), Bügel S(4), Jakobsen J(5). Author information: (1)Research Group for Bioactives - Analysis and Application, National Food Institute, Technical University of Denmark, Henrik Dams Allé, 2800, Kgs. Lyngby, Denmark. (2)Research Group for Analytical Food Chemistry, National Food Institute, Technical University of Denmark, Henrik Dams Allé, 2800, Kgs. Lyngby, Denmark. (3)Arla Innovation Center, Agro Food Park 19, 8200 Aarhus, Denmark. (4)Section for Nutrition and Health, Department of Nutrition, Exercise, and Sports, University of Copenhagen, Rolighedsvej 26, 1958, Kgs, Frederiksberg C, Denmark. (5)Research Group for Bioactives - Analysis and Application, National Food Institute, Technical University of Denmark, Henrik Dams Allé, 2800, Kgs. Lyngby, Denmark. Electronic address: jeja@food.dtu.dk. As diets shift toward sustainable options, plant-based products are gaining popularity, though dairy remains a key source of essential micronutrients. Nutritional value depends not only on content but also on bioaccessibility. This study evaluated the bioaccessibility of riboflavin, vitamin K, calcium, phosphorus, zinc, and iodine in dairy products, their plant-ba

From Common Pathway to Divergent Diseases: Metabolic Aspects of Inborn Errors of CoA Biosynthesis.

4. J Inherit Metab Dis. 2026 Sep;49(5):e70239. doi: 10.1002/jimd.70239. From Common Pathway to Divergent Diseases: Metabolic Aspects of Inborn Errors of CoA Biosynthesis. Di Meo I(1), Anikster Y(2)(3), Tiranti V(1), Iuso A(4)(5). Author information: (1)Unit of Medical Genetics and Neurogenetics, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy. (2)Gray Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. (3)Metabolic Disease Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat Gan, Israel. (4)Institute of Neurogenomics, Helmholtz Zentrum München, Neuherberg, Germany. (5)Institute of Human Genetics, School of Medicine and Health, Technical University of Munich, Munich, Germany. Coenzyme A (CoA) biosynthesis is a conserved, dynamically regulated pathway essential for mitochondrial energy production, fatty acid oxidation, lipid biosynthesis and protein acylation. Biallelic variants in PANK2, PPCS, PPCDC, and COASY cause rare inborn errors of CoA biosynthesis, associated with markedly different clinical phenotypes: PANK2 and COASY defects predominantly cause neurological disorders within or adjacent to the neurodegeneration with brain iron accumulation (NBIA) spectrum, whereas PPCS and PPCDC deficiencies present mainly as severe early-onset dilated cardiomyopathy. However, COASY variants can also cause pontocerebellar hypoplasia and riboflavin-responsive lipid storage myopathy. This review examines

Grape-Derived Exosome-like Nanoparticles Ameliorate Prediabetes in Mice via Amino Acid Metabolic Reprogramming.

5. Int J Mol Sci. 2026 Aug 2;27(15):6947. doi: 10.3390/ijms27156947. Grape-Derived Exosome-like Nanoparticles Ameliorate Prediabetes in Mice via Amino Acid Metabolic Reprogramming. Liu Z(1), Cai S(1), Qiayimaerdan A(1), Tang Y(1), Su J(1), Yang Z(1). Author information: (1)Department of Epidemiology and Health Statistics, School of Public Health, Xinjiang Medical University, Urumqi 830011, China. This study investigates the effects of grape-derived exosomes on prediabetic mice and elucidates their underlying mechanisms through an integrated metabolomics approach. A prediabetic mouse model was established, with 18 mice randomly assigned to three groups: model control group, grape exosome treatment group, and antioxidant nutrient supplementation group. Therapeutic efficacy was assessed using fasting plasma glucose (FPG) and homeostasis model assessment of β-cell function (HOMA-β). Intestinal content metabolites were profiled via both untargeted ultra-high performance liquid chromatography coupled with orbitrap electrospray mass spectrometry (UHPLC-OE-MS) and targeted metabolomics analyses. Grape exosomes effectively mitigated prediabetes progression. Untargeted metabolomics revealed 1222 downregulated and 425 upregulated metabolites in the grape exosome group relative to the model control group, among which riboflavin (upregulated) was potentially associated with amino acid metabolism. Targeted metabolomics identified 23 upregulated and 41 downregulated metab

Dietary Adequacy and Its Associated Factors Among Adolescents in a Rural District of Sindh.

6. Nutrients. 2026 Jul 26;18(15):2438. doi: 10.3390/nu18152438. Dietary Adequacy and Its Associated Factors Among Adolescents in a Rural District of Sindh. Jawad T(1), Rehman N(2), Hashmi M(2), Rizvi A(2), Padhani ZA(3), Gulzar S(4), Farooq H(5), Bux R(2), Chauhadry IA(5)(6), Das JK(2)(5). Author information: (1)Department of Community Health Sciences, Aga Khan University, Karachi 74800, Pakistan. (2)Institute for Global Health and Development, Aga Khan University, Karachi 74800, Pakistan. (3)School of Public Health, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, SA 5005, Australia. (4)School of Nursing and Midwifery (SoNaM), Aga Khan University, Karachi 74800, Pakistan. (5)Department of Pediatrics and Child Health, Aga Khan University, Karachi 74800, Pakistan. (6)Centre of Excellence in Women and Child Health, Aga Khan University, Karachi 74800, Pakistan. Background: Adolescents in low- and middle-income countries face a disproportionate burden of dietary inadequacy, yet evidence on the determinants of overall diet quality remains scarce. This study assessed the prevalence of macro- and micronutrient inadequate intake and identified factors associated with dietary adequacy among adolescents in rural Pakistan. Methods: A cross-sectional survey was conducted among adolescents using multistage cluster sampling. Dietary intake was assessed through a single 24-h dietary recall; nutrient adequacy was determined using age-and sex-specif

Electrochemical reduction degradation and defluorination of perfluorooctane sulfonate mediated by active hydrogen via proton-coupled electron transfer.

7. Water Res. 2026 Aug 4;307:126627. doi: 10.1016/j.watres.2026.126627. Online ahead of print. Electrochemical reduction degradation and defluorination of perfluorooctane sulfonate mediated by active hydrogen via proton-coupled electron transfer. Song J(1), Zeng L(2), Li M(3), Li J(4), Wu Q(4), Han W(5), Liu Z(5), Zhang H(6), Papakonstantinou I(1). Author information: (1)Department of Electronic and Electrical Engineering, University College London, London, UK. (2)School of Environmental Science and Engineering, Guangzhou University-Linköping University Research Center on Urban Sustainable Development, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, 510006, PR China. (3)School of Environmental Science and Engineering, Guangzhou University-Linköping University Research Center on Urban Sustainable Development, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, 510006, PR China; Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, PR China; Guizhou Provincial Key Laboratory for Prevention and Control of Emerging Contaminants, Guizhou University, 2708 Huaxi Road, Guiyang, 550025, PR China. Electronic address: mengli@gzhu.edu.cn. (4)Department of Chemical and Biological Engineering, The Hong Kong University o

Serum Metabolomics Unveils Novel Biomarkers Predicting Efficacy of Dapoxetine: A Prospective Study on Premature Ejaculation.

8. Andrology. 2026 Aug 12. doi: 10.1111/andr.70360. Online ahead of print. Serum Metabolomics Unveils Novel Biomarkers Predicting Efficacy of Dapoxetine: A Prospective Study on Premature Ejaculation. Hou G(1)(2), Zheng Y(2)(3), Meng P(2), Feng X(1), Hou N(4), Federici M(5), Wang F(2), Zhang B(1), Jannini EA(5), Zhang S(6), Zhang G(1), Yuan J(2). Author information: (1)Department of Urology, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. (2)Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. (3)Department of Nephrology, Xijing Hospital, Fourth Military Medical University, Xi'an, China. (4)Department of General Surgery, Air Force 986(Th) Hospital, Fourth Military Medical University, Xi'an, China. (5)Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy. (6)Department of Nuclear Medicine, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. BACKGROUND: As the only approved oral medication for premature ejaculation (PE), dapoxetine faces a high discontinuation rate, primarily due to lower than expected efficacy. The impact of serum metabolites on PE treatment remains undetermined; therefore, we aimed to identify metabolites associated with dapoxetine efficacy. METHODS: Clinical data and blood samples were collected from 116 patients with lifelong PE before 8 weeks of dapoxetine treatment. Serum was analyzed by untargeted metabolomics profiling. Efficacy was assessed with

Vitamin B2 Derivative-Enabled Sonochemical Thioketal Cleavage for Ultrasound-Activated In Situ Tumor Vaccination.

9. Nano Lett. 2026 Aug 12;26(31):10413-10424. doi: 10.1021/acs.nanolett.6c02398. Vitamin B2 Derivative-Enabled Sonochemical Thioketal Cleavage for Ultrasound-Activated In Situ Tumor Vaccination. Ren X(1)(2)(3), Sun J(4), Cui L(1)(3), Xu Y(1)(3), Shen N(1)(3), Tang Z(1)(2)(3), Chen X(1)(2)(3). Author information: (1)State Key Laboratory of Polymer Science and Technology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin130022, China. (2)School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui230026, China. (3)Jilin Biomedical Polymers Engineering Laboratory, Changchun, Jilin130022, China. (4)Key Laboratory of Zoonosis Research, Ministry of Education, College of Basic Medical Science, Jilin University, Changchun130021, Jilin, China. Toll-like receptor (TLR) agonists are promising for in situ tumor vaccination but suffer from poor tumor selectivity and systemic toxicity. Here, we establish a sonochemical activation strategy using a vitamin B2-derived sonosensitizer for spatiotemporally controlled immunotherapy. Riboflavin tetrabutyrate (TBR) was identified as an efficient sonosensitizer for thioketal (TK) cleavage under low-intensity ultrasound (US) and coformulated with a TK-masked TLR7/8 agonist (R848) to construct sonosensitive nanoparticles (R848/TBR NPs). Upon US irradiation, TBR generated singlet oxygen to induce immunogenic cell death and trigger R848 release through a

Genome-wide meta-analysis with 1,590,596 individuals identifies 12 novel risk loci for systemic lupus erythematosus.

10. Mamm Genome. 2026 Aug 11;37(1):95. doi: 10.1007/s00335-026-10266-9. Genome-wide meta-analysis with 1,590,596 individuals identifies 12 novel risk loci for systemic lupus erythematosus. Zhu G(#)(1)(2), Su W(#)(1)(2), Deng J(#)(2)(3)(4), Zhai B(#)(5), Gao R(1)(2), Qiu J(1)(2), Gu K(1)(2), Bian Z(1)(2), Xiao H(6), Luan G(7)(8)(9), Wang R(10)(11). Author information: (1)Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Collaborative Innovation Center for Brain Disorders, Ministry of Science and Technology, Capital Medical University, Beijing, 100069, China. (2)Laboratory for Clinical Medicine, Capital Medical University, Beijing, 100069, China. (3)Department of Neurosurgery, SanBo Brain Hospital, Capital Medical University, Beijing, 100089, China. (4)Beijing Key Laboratory of Epilepsy Research, Department of Brain Institute, Center of Epilepsy, Beijing Institute for Brain Disorders, Sanbo Brain Hospital, Capital Medical University, Beijing, 100089, China. (5)Department of Hematology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, 100039, China. (6)State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China. (7)Laboratory for Clinical Medicine, Capital Medical University, Beijing, 100069, China. luangm@ccmu.edu.cn. (8)Department of Neurosurgery, SanBo Brain Hospital, Capital Medical Univers

Fluorescence Anisotropy of Riboflavin vis-à-vis Protochlorophyllide in Lemon Juice.

11. J Fluoresc. 2026 Aug 11. doi: 10.1007/s10895-026-04901-6. Online ahead of print. Fluorescence Anisotropy of Riboflavin vis-à-vis Protochlorophyllide in Lemon Juice. Basumatary T(1), Borah AJ(2), Barua AG(3). Author information: (1)Department of Physics, Gauhati University, Guwahati, 781014, India. (2)Department of Physics, Gauhati University, Guwahati, 781014, India. amlanjyoti001@gmail.com. (3)Department of Physics, Gauhati University, Guwahati, 781014, India. agb@gauhati.ac.in. A violet laser of wavelength 405 nm is used to excite steady-state fluorescence in the juice of a lemon. Prominent wavelength peaks appear at 520 and 670 nm due to emissions from riboflavin (vitamin B2) and protochlorophyllide (pchlide), the precursor of chlorophyll [Formula: see text], respectively. Using a polariser for excitation with vertically polarised light, and another one in parallel as well as in perpendicular positions to this one in the emission-path, counts at the two peaks in the high-resolution spectrometer are noted. Fluorescence anisotropies calculated from the obtained intensities at the two positions of the analyser reveal that the value at 520 nm peak is approximately twice that of the 670 nm one. This indicates that rotational mobility of riboflavin is much less than protochlorophyllide, even though the latter is much larger and more complex, in the low pH (high acidity) medium. © 2026. The Author(s), under exclusive licence to Springer Science+Business Me

Identification of pulmonary intersegmental planes using the intravenous administration of vitamin B2 with a blacklight.

12. Surg Today. 2026 Aug 10. doi: 10.1007/s00595-026-03442-6. Online ahead of print. Identification of pulmonary intersegmental planes using the intravenous administration of vitamin B2 with a blacklight. Saito D(1), Yuki H(1), Nishikawa S(1), Takahashi T(1), Terada Y(1), Kakegawa S(1), Matsumoto I(2). Author information: (1)Department of Thoracic Surgery, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-8641, Ishikawa, Japan. (2)Department of Thoracic Surgery, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-8641, Ishikawa, Japan. isa-mat@med.kanazawa-u.ac.jp. The precise identification of intersegmental planes is critical in lung segmentectomy. Indocyanine green (ICG) is commonly used, but it requires specialized equipment and it also has a short fluorescence duration. This study explored the use of intravenous vitamin B2 (riboflavin) with a blacklight (ultraviolet A, UV-A) as a simpler, cost-effective alternative. In a porcine model (n = 4), both ICG and vitamin B2 accurately delineated the same intersegmental plane. However, vitamin B2 fluorescence lasted over 30 min -significantly longer than ICG's 7.3 min -and it was visible to the naked eye without specialized imaging systems. The method was safe and practical, utilizing readily available UV light. This approach may be particularly useful for visualizing lung segments during surgery without the need for expensive fluorescence cameras. Although the results in healthy animals are promising, fu

Stromal Lenticule Addition Keratoplasty (SLAK) for the Management of Severe Corneal Flattening After Corneal Cross-linking.

13. J Refract Surg. 2026 Aug;42(8):e852-e856. doi: 10.3928/1081597X-20260629-01. Epub 2026 Aug 1. Stromal Lenticule Addition Keratoplasty (SLAK) for the Management of Severe Corneal Flattening After Corneal Cross-linking. Kymionis GD(1), Tsagkogiannis VA(1), Vakalopoulos DG(1), Petrou PC(1). Author information: (1)First Department of Ophthalmology, National and Kapodistrian University of Athens, "G. Gennimatas" Hospital, Athens, Greece. PURPOSE: To report the first case of stromal lenticule addition keratoplasty performed for the management of severe progressive corneal flattening following corneal cross-linking (CXL). METHODS: A 63-year-old woman with keratoconus, previously treated with combined topography-guided photorefractive keratectomy and CXL (Athens protocol), presented with progressive corneal flattening and a hyperopic shift in the left eye. Corrected distance visual acuity (CDVA) was 20/50 with manifest refraction of +5.00 -1.50 × 123° and the mean keratometry was 36.20 diopters (D). Stromal lenticule addition keratoplasty was performed using a stromal lenticule obtained from a myopic small incision lenticule extraction treatment. A femtosecond laser was used to create an intrastromal pocket, and the lenticule was inserted and centered within the stromal interface. RESULTS: Postoperatively, mean keratometry increased to 41.20 D and refraction improved to +0.50 -2.00 × 110°, with 20/40 CDVA. Corneal curvature remained stable at 12 months postoper

Oral Riboflavin for Sunlight-Induced Corneal Cross-linking: Efficacy Assessment in a Rabbit Model In Vivo Using Extensometry and OCT Elastography.

14. J Refract Surg. 2026 Aug;42(8):e797-e803. doi: 10.3928/1081597X-20260622-02. Epub 2026 Aug 1. Oral Riboflavin for Sunlight-Induced Corneal Cross-linking: Efficacy Assessment in a Rabbit Model In Vivo Using Extensometry and OCT Elastography. Torres-Netto EA(1)(2), Aydemir ME(1), Lu NJ(1)(3)(4), Kling S(5)(6), Hafezi N(1)(7), Hillen M(1), Depczynska ML(1), Hafezi F(1)(2)(3)(8)(9)(10). Author information: (1)ELZA Institute, Zurich, Switzerland. (2)Laboratory of Ocular Cell Biology, Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Switzerland. (3)Department of Ophthalmology, Medical University of Wenzhou, Wenzhou, China. (4)Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu, China. (5)Computer Vision Laboratory, Department of Information Technology and Electrical Engineering, Swiss Federal Institute of Technology, Zurich, Switzerland. (6)ARTORG Center for Biomedical Engineering Research, University of Bern, Bern, Switzerland. (7)Department of Ophthalmology, Antwerp University Hospital, Edegem, Belgium. (8)Faculty of Medicine, University of Geneva, Geneva, Switzerland. (9)USC Roski Eye Institute, University of Southern California, Los Angeles, California and. (10)Department of Ophthalmology, New York University Grossman School of Medicine, New York, New York. PURPOSE: To evaluate whether a non-invasive approach to treat keratoconus with corneal cross-linking (CXL) using oral riboflavin and natural sunlight

Mare milk and its extracellular vesicles alleviate DSS-induced colitis in mice by modulating immune responses and reshaping gut microbiota.

15. Food Funct. 2026 Aug 10. doi: 10.1039/d6fo01504b. Online ahead of print. Mare milk and its extracellular vesicles alleviate DSS-induced colitis in mice by modulating immune responses and reshaping gut microbiota. Si Q(1), Wu Y(2), Bao X(3), Ta L(4), Bao J(4), Siren B(4), Byambaa T(5), Shi J(6), Li X(7), Jiao J(1)(6). Author information: (1)Research Department, Inner Mongolia International Hospital of Mongolian Medicine, Hohhot, China. (2)Pharmacy Department, Inner Mongolia International Hospital of Mongolian Medicine, Hohhot, China. (3)Medical Innovation Center for Nationalities, Inner Mongolia Medical University, Hohhot, China. (4)School of Mongolian Medicine and Pharmacy, Inner Mongolia Medical University, Hohhot, China. (5)Department of Traditional Prescriptionology, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. (6)School of Traditional Chinese Medicine, Inner Mongolia Medical University, Hohhot, China. (7)School of Basic Medicine, Inner Mongolia Medical University, Hohhot, China. Ulcerative colitis (UC) is a chronic inflammatory disorder driven by gut dysbiosis and immune dysfunction. This study evaluated the protective effects of mare milk (MM) and its extracellular vesicles (MM-EVs) in DSS-induced UC. Both interventions alleviated clinical symptoms, restored colonic architecture, and rebalanced mucosal and systemic T-cell immunity. MM preferentially promoted colonic Th17 responses, whereas MM-EVs selectively suppressed Th

A photo-crosslinkable hyaluronic acid-collagen hydrogel maintains epithelial integrity and supports intestinal organoid stemness.

1. Regen Biomater. 2026 Aug 7;13:rbag171. doi: 10.1093/rb/rbag171. eCollection 2026. A photo-crosslinkable hyaluronic acid-collagen hydrogel maintains epithelial integrity and supports intestinal organoid stemness. Cho Y(1), Park SY(2), Han DW(3), Lee HJ(2), Lee JH(1). Author information: (1)Department of Food Science and Biotechnology, Gachon University, Seongnam-si 13120, Republic of Korea. (2)School of Chemical, Biological and Battery Engineering, Gachon University, Seongnam-si 13120, Republic of Korea. (3)Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 46241, Republic of Korea. Organoids provide physiologically relevant platforms for disease modeling and drug screening, but their performance depends critically on the extracellular matrix (ECM). Matrigel, the current standard, suffers from undefined composition, batch-to-batch variability and limited tunability. We engineered a photo-crosslinkable hyaluronic acid-collagen (HA-collagen) composite hydrogel using riboflavin-mediated blue-light activation and examined how the collagen-to-HA ratio modulates physicochemical and biological properties. Storage modulus scaled with HA content (Col4HA1: 156 Pa; Col3HA1: 216 Pa; Col2HA1: 824 Pa vs. 6 Pa for Matrigel), and organoid formation efficiency was inversely correlated with stiffness: Col2HA1 yielded significantly fewer organoids than Matrigel, whereas Col4HA1 fell within a mechanically permissive range and supported organoid f

Multilevel metabolic engineering of Shewanella oneidensis as a heme-tolerant host for efficient porphyrin production.

2. Metab Eng. 2026 Oct 6:102560. doi: 10.1016/j.ymben.2026.102560. Online ahead of print. Multilevel metabolic engineering of Shewanella oneidensis as a heme-tolerant host for efficient porphyrin production. Sun D(1), Wu J(2), He RL(3), Wu J(1), Lin WQ(1), Zhao HP(4), Liu DF(5), Li WW(6). Author information: (1)School of Life Sciences, University of Science and Technology of China, Hefei, 230026, China. (2)College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China. (3)Institute of Environment Hefei Comprehensive National Science Center, Hefei 230026, China. (4)State Key Laboratory of Soil Pollution Control and Safety, Zhejiang University, Hangzhou, 310058, China. (5)State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, 230026, China. Electronic address: dfl@ustc.edu.cn. (6)State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, 230026, China. Electronic address: wwli@ustc.edu.cn. Porphyrin-derived high-value chemicals are important for food, medicine, and fine chemicals manufacturing, yet the physiological complexity of tetrapyrrole metabolism constrains efficient bioproduction. Here, we systematically engineered the cytochrome-rich bacterium Shewanella oneidensis MR-1 for porphyrin production. A modular coproporphyrin III biosynthetic p

METABOLOMIC PROFILING-BASED CHEMOTAXONOMIC EVALUATION OF THE GENUS IBERIS L. (BRASSICACEAE) IN TÜRKİYE.

3. Planta Med. 2026 Oct 4. doi: 10.1055/a-2973-6912. Online ahead of print. METABOLOMIC PROFILING-BASED CHEMOTAXONOMIC EVALUATION OF THE GENUS IBERIS L. (BRASSICACEAE) IN TÜRKİYE. Zare G(1), Diker NY(1), Kaplan O(2), Çilden E(3), Çankaya İİ(1). Author information: (1)Hacettepe University, Faculty of Pharmacy, Department of Pharmaceutical Botany, Ankara, Turkey, Ankara. (2)Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry, Turkey, Ankara. (3)Hacettepe University, Faculty of Science, Department of Biology, Turkey, Ankara. The genus Iberis L. is mainly distributed in the Mediterranean basin, with Europe representing the primary center of diversity, while a limited number of species occur in Northwest Africa and Southwest to Central Asia. The genus is represented by ten taxa in Türkiye, including four endemic taxa: Iberis carica (Bornm.) Prain, Iberis halophila Vural & H. Duman, Iberis gypsicola Yıld. and Iberis saxatilis subsp. Magnesiana Oskay. Previous studies emphasized morphology and phylogeny, whereas comprehensive metabolomic and phytochemical analyses of the genus remain limited. In this study, plant materials from prior morphological and molecular analyses underwent LC-MS-based untargeted metabolomic profiling to provide complementary chemotaxonomic evidence and evaluate how closely metabolomic patterns align with other taxonomic datasets. A total of 149 LC-MS features were putatively annotated. Primary metabolites showed h

Effect of chitosan-functionalized ferritin-riboflavin nanoparticles as a novel vitamin delivery system on Pickering emulsions: Rheology, stability, and in vitro release kinetics.

4. Int J Biol Macromol. 2026 Oct 3:154703. doi: 10.1016/j.ijbiomac.2026.154703. Online ahead of print. Effect of chitosan-functionalized ferritin-riboflavin nanoparticles as a novel vitamin delivery system on Pickering emulsions: Rheology, stability, and in vitro release kinetics. Xu Q(1), Cao Y(2), Wang X(1), Luo R(1), Wang S(1). Author information: (1)College of Food Science and Engineering, Ningxia University, Yinchuan, China. (2)College of Food Science and Engineering, Ningxia University, Yinchuan, China. Electronic address: caoyj035@163.com. This work aimed to investigate the feasibility and delivery capacity of Pickering emulsions stabilized by chitosan-modified self-assembled ferritin-riboflavin nanoparticles (BRC-NPs). Results showed that after the modification of nanoparticles with chitosan, the size of BRC-NPs was 56.99 nm. The structure, thermal and functional properties of the BRC-NPs were significantly improved through the electrostatic interaction between chitosan and ferritin. Rheological properties, emulsifying properties and stability of the Pickering emulsion were modulated by BRC-NPs concentration. When the BRC-NPs concentration was 0.4%, the Pickering emulsion had better viscosity, viscoelasticity and creep properties. Moreover, 0.4% BRC-NPs can increase the storage and light stability of the emulsion and delay its lipid oxidation. In vitro digestion results showed that ferritin digestion and lipid oxidation in the emulsion were delayed

Excellent response to high-dose Riboflavin in Brown-Vialetto-Van Laere syndrome: A case report.

5. Medicine (Baltimore). 2026 Oct 2;105(40):e51027. doi: 10.1097/MD.0000000000051027. Excellent response to high-dose Riboflavin in Brown-Vialetto-Van Laere syndrome: A case report. Ali A(1), Abdullah A(2), Masood HMY(1), Saleem U(2), Talha M(2), Ghafoor LA(1), Sharma MK(3). Author information: (1)Department of Neurology, Allied Hospital Faisalabad, Faisalabad, Pakistan. (2)Department of Internal Medicine, Allied Hospital Faisalabad, Faisalabad, Pakistan. (3)Department of Neurology, Dhankuta Multiple Campus, Tribhuvan University, Nepal. RATIONALE: Brown-Vialetto-Van Laere syndrome, currently recognized within the spectrum of riboflavin transporter deficiency type 3 (RTD3), is a rare inherited neurometabolic disorder associated with pathogenic variants in SLC52A3. It typically presents with progressive sensorineural hearing loss, pontobulbar dysfunction, peripheral neuropathy, and respiratory involvement. Diagnosis may be delayed because routine metabolic and neuromuscular investigations can be unrevealing despite a characteristic clinical phenotype. PATIENT CONCERNS: A young Asian man presented with several years of progressive bilateral hearing loss followed by worsening dysphagia to solids and liquids, symmetrical limb weakness, muscle wasting, and acute-on-chronic dyspnea. Two siblings had previously died at a young age after developing a similar sequence of hearing impairment, dysphagia, limb weakness, and unexplained respiratory failure. DIAGNOSES: Ne

The riboflavin kinase domain is essential for FMNAT catalysis: A linker-dependent regulatory mechanism in Staphylococcus aureus FAD synthetase.

6. Biochimie. 2026 Oct 1:S0300-9084(26)00235-X. doi: 10.1016/j.biochi.2026.09.016. Online ahead of print. The riboflavin kinase domain is essential for FMNAT catalysis: A linker-dependent regulatory mechanism in Staphylococcus aureus FAD synthetase. Sahana R(1), Bakshi A(2), Kumar S(3), Lohithakshan A(1), Usharani D(2), Nandini C(4), Kumar R(5). Author information: (1)Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, 570020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. (2)Department of Food Safety and Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, 570020, India. (3)School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India. (4)Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, 570020, India. (5)School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: ravikr@jnu.ac.in. Flavin adenine dinucleotide (FAD) synthetase (FADS) from Staphylococcus aureus is a bifunctional enzyme comprising an N-terminal FMN adenylyltransferase (FMNAT) domain that catalyzes FAD synthesis and a C-terminal riboflavin kinase (RFK) domain responsible for riboflavin phosphorylation. While eukaryotic FMNAT and RFK homologs function as independently, the mechanistic basis for their fusion and

Characterization of FAD binding to the apo-form of diflavin-linked disulfide oxidoreductase derived from cyanobacterium Synechocystis sp. PCC6803.

7. Biochem J. 2026 Oct 1:BCJ20260320. doi: 10.1042/BCJ20260320. Online ahead of print. Characterization of FAD binding to the apo-form of diflavin-linked disulfide oxidoreductase derived from cyanobacterium Synechocystis sp. PCC6803. Hirano Y(1), Suzuki T(2), Umeda N(2), Minato Y(2), Yoshimura K(2), Tamada T(1), Kimura S(3). Author information: (1)National Institutes for Quantum Science and Technology, Chiba, Japan. (2)Ibaraki Daigaku Kogakubu, Ibaraki, Japan. (3)Ibaraki University, Hitachi, Japan. Diflavin-linked disulfide oxidoreductase (DDOR) from the cyanobacterium Synechocystis sp. PCC6803 is an NADPH-thioredoxin reductase (NTR) superfamily enzyme that forms a homodimer containing two FAD molecules (FAD1 and FAD2) per monomer. FAD1 is conserved in the NTR superfamily, while FAD2 is characteristic of DDOR. Under aerobic conditions, thiol reduction of DDOR produces an apparently air-stable neutral blue semiquinone, but the respective roles of FAD1 and FAD2 in the reduction process remain unclear. To address this, spectroscopic, structural and mutational analyses were performed to investigate the contributions of the two FAD molecules. Recombinant apo- and holo-DDOR synthesised in Escherichia coli cells were separated and purified. Fluorescence titration of the apo-DDOR with FAD revealed rapid binding of two FADs. Stopped-flow analysis indicated biphasic binding of FAD to apo-DDOR. X-ray crystal structures of apo- and holo-DDORs exhibited pronounced confo

Photocatalytic synthesis of phosphorothioates by sulfur transfer from thiosulfate.

8. Chem Sci. 2026 Sep 22. doi: 10.1039/d6sc06072b. Online ahead of print. Photocatalytic synthesis of phosphorothioates by sulfur transfer from thiosulfate. Budeev A(1), Bon C(1), Sparr C(1). Author information: (1)Department of Chemistry, University of Basel St. Johanns-Ring 19 Basel 4056 Switzerland christof.sparr@unibas.ch. With therapeutic oligonucleotides progressing from the treatment of rare genetic diseases to the medication of common disorders, the synthesis of modified oligonucleotides is gaining considerable significance. To improve pharmacokinetic and pharmacodynamic properties, chemical modifications to the nucleotide backbone are thereby especially advantageous. In particular, the incorporation of phosphorothioate moieties has proven to be an effective strategy, since this linkage often provides increased metabolic stability and beneficial activity compared to phosphates. Herein, we describe that visible-light photocatalysis allows the generation of phosphorothioates by an exceptionally mild and general sulfurization with conventional thiosulfate as a benign sulfur source. The method was applied to efficiently prepare DNA and RNA phosphorothioates, phosphoramidothioate, phosphine sulfides and phosphoramidodithioate. Furthermore, the protocol was translated to the synthesis of phosphoroselenoates using selenosulfate and was integrated into standard solid-phase phosphoramidite oligonucleotide synthesis to form phosphorothioates with riboflavin

Relative Validity of Food Frequency Questionnaires in Adult Populations: A Systematic Review and Meta-Analysis.

9. Food Sci Nutr. 2026 Sep 30;14(10):e72381. doi: 10.1002/fsn3.72381. eCollection 2026 Oct. Relative Validity of Food Frequency Questionnaires in Adult Populations: A Systematic Review and Meta-Analysis. Pujia C(1), Ferro Y(2), Placanica A(3), Bruno R(2), Guadagni F(4), Malaguarnera G(4), Mazza E(3), Montalcini T(3)(5), Pujia A(2)(5). Author information: (1)O.U. Clinical Nutrition Renato Dulbecco Hospital Catanzaro Italy. (2)Department of Medical and Surgical Science University Magna Grecia Catanzaro Italy. (3)Department of Clinical and Experimental Medicine University Magna Grecia Catanzaro Italy. (4)Department of Human Sciences and Quality of Life Promotion San Raffaele Roma Open University Rome Italy. (5)Research Center for the Prevention and Treatment of Metabolic Diseases University Magna Grecia Catanzaro Italy. Accurate dietary assessment is essential in nutritional research and epidemiology. Food Frequency Questionnaires (FFQs) are widely used to estimate habitual intake; however, their performance may vary according to questionnaire design, administration procedures, and the dietary components being assessed. This systematic review and meta-analysis evaluated the relative validity of FFQs designed to assess overall dietary intake in adults and explored methodological characteristics associated with their performance. A meta-analysis was conducted on studies validating FFQs against reference methods, including 24-h recalls and food records. A compreh

Mycobacterium tuberculosis requires riboflavin biosynthesis for survival in the host.

10. bioRxiv [Preprint]. 2026 Sep 26:2026.09.25.754416. doi: 10.64898/2026.09.25.754416. Mycobacterium tuberculosis requires riboflavin biosynthesis for survival in the host. Block AM, Namugenyi SB, Tischler AD. Mycobacterium tuberculosis has a flavin-intensive metabolism, with more than 180 proteins that require flavin or deazaflavin cofactors. M. tuberculosis requires riboflavin biosynthesis for growth in standard culture conditions in vitro , which can be overcome by providing exogenous riboflavin. It is unknown whether M. tuberculosis also requires riboflavin synthesis to grow or persist in infected host cells or tissues. Here we used a M. tuberculosis strain that conditionally expresses ribA2 , which encodes a bifunctional enzyme that catalyzes the first steps in both branches of riboflavin biosynthesis, to determine whether M. tuberculosis requires riboflavin biosynthesis during infection. Transcriptional repression of ribA2 caused death of M. tuberculosis in vitro without riboflavin supplementation. RNAseq revealed that riboflavin starved M. tuberculosis induce a transcriptional program indicating decreased electron transport chain function and broad changes to central metabolism. Transcriptional repression of ribA2 prevented M. tuberculosis growth in cultured macrophages and caused clearance of M. tuberculosis from infected tissues at all stages of infection in both C57BL/6 mice that form cellular lung lesions and C3HeB/FeJ mice that develop necrot

Perfusate Flavin Mononucleotide and D-Dimer as Viability Biomarkers in Australasia's First Hypothermic Machine Perfusion Programme for Liver Transplantation.

11. Clin Transplant. 2026 Oct;40(10):e70697. doi: 10.1111/ctr.70697. Perfusate Flavin Mononucleotide and D-Dimer as Viability Biomarkers in Australasia's First Hypothermic Machine Perfusion Programme for Liver Transplantation. Eastment J(1)(2), Devaux J(3)(4), Yi K(1)(5), Swan P(1)(5), McCall J(1)(5), Jones A(6), Barbier L(1)(5). Author information: (1)New Zealand Liver Transplant Unit, Auckland City Hospital, Auckland, New Zealand. (2)Department of Surgery, School of Medicine, The University of Queensland, Brisbane, Queensland, Australia. (3)Applied Surgery and Metabolism Laboratory, University of Auckland, Auckland, New Zealand. (4)Institute For Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia. (5)Department of Surgery, School of Medicine, University of Auckland, Auckland, New Zealand. (6)Queensland Digital Health Centre, The University of Queensland, Brisbane, Queensland, Australia. Hypothermic oxygenated machine perfusion (HOPE) is a simple ex-situ treatment for liver grafts. However, it lacks viability assessment capabilities. Flavin mononucleotide (FMN) and D-dimer may be useful biomarkers of graft quality during HOPE. This exploratory study aimed to determine whether effluent FMN and D-dimer concentrations were associated with outcomes in liver transplants treated with HOPE. This is a retrospective, single-center study of all grafts treated with HOPE in New Zealand from 2022-2025. Fifty-four grafts were treated wit

Neonatal high-dose riboflavin attenuates abdominal adiposity and binge eating associated with adipose metabolic remodeling in experimental cerebral palsy.

12. Nutr Neurosci. 2026 Sep 30:1-23. doi: 10.1080/1028415X.2026.2738370. Online ahead of print. Neonatal high-dose riboflavin attenuates abdominal adiposity and binge eating associated with adipose metabolic remodeling in experimental cerebral palsy. Silva-Araújo ER(1)(2)(3)(4), Manhães-de-Castro R(1)(2)(5), Dos Santos Júnior OH(1)(2), Dos Santos Júnior JP(2)(5), Antônio Da Silva Araújo M(1)(2), José Cavalcanti Bezerra Gouveia H(6), De Oliveira Rodrigues T(1)(2), Geneilson Silva J(2), Jimenez-Chillaron JC(3), Padrón-Hernández E(1)(4)(7), Toscano AE(1)(2)(5)(8). Author information: (1)Graduate Program in Neuropsychiatry and Behavioral Sciences, Center for Medical Sciences, Federal University of Pernambuco, Recife-Pernambuco, Brazil. (2)Studies in Nutrition and Phenotypic Plasticity Unit, Center for Health Sciences, Federal University of Pernambuco, Recife-Pernambuco, Brazil. (3)Biophysics Unit, Department of Physiological Sciences II, School of Medicine, University of Barcelona, Barcelona, Spain. (4)Laboratory of Mesomagnetics, Physics Department, Center of Exact and Natural Sciences, Federal University of Pernambuco, Recife-Pernambuco, Brazil. (5)Graduate Program in Nutrition, Center for Health Sciences, Federal University of Pernambuco, Recife-Pernambuco, Brazil. (6)Department of Anatomy, Université du Québec à Trois-Rivières, Trois-Rivières, Canada. (7)Physics Department, Center of Exact and Natural Sciences, Federal University of Pernambuco, Recife-Pernambu

Gut and skin microbiome-metabolome pathways in vitiligo: from dysbiosis to immune activation and melanocyte dysfunction.

13. Front Immunol. 2026 Sep 15;17:1952635. doi: 10.3389/fimmu.2026.1952635. eCollection 2026. Gut and skin microbiome-metabolome pathways in vitiligo: from dysbiosis to immune activation and melanocyte dysfunction. Ruiz-Pozo VA(#)(1), Tamayo-Trujillo R(#)(1), Cadena-Paredes AS(1), Guevara-Ramírez P(1), Cadena-Ullauri S(1), Paz-Cruz E(1), Zambrano AK(1). Author information: (1)Centro de Investigación Genética y Genómica, Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito, Ecuador. (#)Contributed equally Vitiligo is an autoimmune depigmenting disorder driven by genetic susceptibility, oxidative stress, immune dysregulation, and progressive melanocyte loss. Emerging microbiome-metabolome evidence suggests that gut and skin dysbiosis may contribute to disease progression through the gut-skin axis. This review integrates current evidence linking microbial imbalance with immune activation and melanocyte dysfunction in vitiligo. Studies in patients and experimental vitiligo models show altered gut and skin microbial diversity, enrichment of inflammation-associated taxa, depletion of commensal bacteria, impaired short-chain fatty acid biosynthesis, and disruption of tryptophan-indole, kynurenine, bile acid, taurine, riboflavin, and oxidative stress-related metabolic pathways. These alterations may weaken epithelial barrier integrity, impair immune tolerance, promote systemic low-grade inflammation, and increase melanocyte vulnerability to mito

Label-free optical biomarker for prostate cancer diagnosis.

14. Mol Med. 2026 Aug 17;32(1):165. doi: 10.1186/s10020-026-01605-2. Label-free optical biomarker for prostate cancer diagnosis. Alam SR(1), Siller K(2), Dreicer R(3), Periasamy A(4)(5). Author information: (1)Department of Biology and W.M. Keck Center for Cellular Imaging, Charlottesville, USA. (2)Research Computing and School of Data Science, Charlottesville, USA. (3)Division of Hematology & Oncology, UVA Comprehensive Cancer Center, Charlottesville, USA. (4)Department of Biology and W.M. Keck Center for Cellular Imaging, Charlottesville, USA. ap3t@virginia.edu. (5)Department of Biomedical Engineering, University of Virginia, Charlottesville, USA. ap3t@virginia.edu. BACKGROUND: Reprogramming of cellular metabolism in cancer leads to changes in the activity of metabolic coenzymes NAD(P)H and FAD which can be used as markers of tumorigenesis and metastasis. NAD(P)H and FAD form coupled redox pair and regulator of mitochondrial energy metabolism. Since NAD(P)H and FAD are endogenous and auto fluorescent, the coupled redox pair can hence be exploited as a reporter for metabolic activity in tumor microenvironment. METHODS: We have developed a 2P-FLIRR assay (2-photon Fluorescence Lifetime based Redox Ratio), a sensitive metabolic optical biomarker for tumor diagnosis in prostate cancer (PCa) patients' tissue sections. We have imaged and used our novel 2P-FLIRR metric which is the ratio of the enzyme-bound fractions of NAD(P)H and FAD to evaluate PCa patients' su

Lactobacillus and Tryptophan Supplementation Improve Renal Function and are Associated with Metabolomic Alterations in a Mouse Model of Chronic Kidney Disease.

15. Probiotics Antimicrob Proteins. 2026 Sep 30. doi: 10.1007/s12602-026-11237-x. Online ahead of print. Lactobacillus and Tryptophan Supplementation Improve Renal Function and are Associated with Metabolomic Alterations in a Mouse Model of Chronic Kidney Disease. Yin M(1), Li M(2), Zhang Y(1), Wang B(1), Li G(1), Wu G(3). Author information: (1)Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. (2)Department of Nephrology, Ninth People's Hospital of Zhengzhou, Zhengzhou, 450053, China. (3)Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. fccwug@zzu.edu.cn. Gut microbiota has been implicated in chronic kidney disease (CKD) progression. Probiotics such as Lactobacillus may alleviate renal injury through metabolic modulation, whereas tryptophan metabolism may have divergent effects in CKD. However, whether Lactobacillus and tryptophan supplementation are associated with distinct or overlapping metabolic profiles in CKD remains unclear. This study aimed to evaluate the effects of Lactobacillus and tryptophan supplementation and characterize the associated serum and fecal metabolomic alterations in an adenine-induced CKD mouse model. Six-week-old male mice were fed adenine for 3 weeks to induce CKD, followed by 8 weeks of treatment: saline for control (NC, CKD), Lactobacillus (2 × 107 CFU; CKD + Lac), or Tryptophan (4 mmol/kg; CKD + Try). Renal func