하수오 (적하수오 · 탈모/새치 및 간독성 주의)

This study aimed to optimize the extraction and purification of TSG-enriched Polygonum multiflorum extract and evaluate its therapeutic potential against androgenetic alopecia. P. multiflorum Thunb. has long been used in traditional Chinese medicine to promote hair growth and preserve hair pigmentation. Microwave-assisted extraction (MAE) was optimized to obtain a 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG)-enriched P. multiflorum extract (PME). The crude extract was further purified by medium-pressure liquid chromatography and semipreparative high-performance liquid chromatography. Phytochemical profiling by HPLC demonstrated a 3.75-fold increase in TSG content, from 22,770.0 ± 815 ppm to 85,387.5 ± 192 ppm, and the identity of the enriched TSG was confirmed by LC-MS and 1H NMR spectroscopy. PME exhibited potent dose-dependent inhibition of 5α-reductase activity, reaching 92.7% inhibition at 1 μg/mL, comparable to that of finasteride. In a testosterone (TES)-induced androgenetic alopecia (AGA) mouse model, PME markedly accelerated hair regrowth and increased both the number and size of hair follicles. Mechanistically, PME suppressed the conversion of TES to dihydrotestosterone (DHT) through 5α-reductase inhibition and promoted GSK3β inactivation, β-catenin stabilization, and nuclear translocation, indicating activation of the Wnt/β-catenin signaling pathway. Consequently, the expression of hair growth-related proteins, including Ki67 (1.98-fold), epidermal growth factor (EGF, 1.54-fold), insulin-like growth factor-1 (IGF-1, 1.21-fold), and vascular endothelial growth factor (VEGF, 1.34-fold), was significant

Seborrheic alopecia (SA) is one of the most common forms of hair loss with a complex pathogenesis involving multiple etiological factors. Although the scalp microbiome has been implicated in various scalp disorders, its specific role in the development and progression of SA remains incompletely understood. To characterize the scalp microbiome in SA, we performed high-throughput sequencing of the 16S rRNA gene and ITS region on scalp samples from 41 Chinese SA participants before and after a 12-week intervention with a shampoo containing herbal extracts (ginger root, Polygonum multiflorum, and Platycladus orientalis leaf) and 29 healthy controls. The untreated SA group exhibited significant microbial dysbiosis compared to the healthy controls, characterized by reduced bacterial and fungal alpha diversity and increased relative abundances of Staphylococcus, Cutibacterium, and Malassezia. LEfSe analysis confirmed the significant enrichment of these three genera. Correlation network analysis revealed a substantial restructuring of microbial interactions in the untreated SA group: Staphylococcus and Malassezia lost all positive correlations with other genera, whereas Cutibacterium displayed relatively stable topological relationships. Following the 12-week intervention, the treated SA group showed significant clinical improvement (reduced hair loss and scalp sebum content), along with a restoration of microbial diversity to levels comparable to the healthy group and a normaliza

INTRODUCTION: Polygonum multiflorum (PM), a traditional medicinal herb, is renowned for its regenerative effects on hair growth; however, its therapeutic application has been largely confined to crude extracts. Recent advances have highlighted plant-derived nanovesicles (PDNVs) as natural carriers of bioactive molecules. This study aimed to evaluate the hair growth-promoting potential of nanovesicles derived from the roots of Polygonum multiflorum (PM-NVs). METHODS: PM-NVs were isolated from fresh PM roots using differential and density gradient ultracentrifugation. Their morphology was confirmed by transmission electron microscopy. Human dermal papilla cells (DPCs) were treated with PM-NVs to assess proliferation using the CCK-8 assay and β-catenin activation via Western blot and quantitative RT-PCR (qRT-PCR). Ex vivo cultured human hair follicles (HFs) were treated with PM-NVs to evaluate hair shaft elongation. RESULTS: PM-NVs were spherical and efficiently internalized by DPCs. Treatment with PM-NVs significantly increased DPC proliferation in a dose-dependent manner, upregulated β-catenin protein levels,

Polygonum multiflorum is a traditional Chinese medicinal herb used to nourish the blood, promote hair growth, and support neurological health. Its key bioactive component, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), has shown potent antioxidant and anti-aging properties, making it a representative marker compound in modern pharmacological studies. We aimed to investigate the neuroprotective effects of THSG against cadmium (Cd)-induced neurotoxicity and to elucidate its underlying molecular mechanisms using both in vitro cellular models and an in vivo murine model. Male mice, 8 weeks of age, were randomly assigned to four groups: control, Cd-exposed, THSG-treated, and Cd+THSG co-treated. After 30 days of Cd exposure with or without THSG, behavioral tests, histological analysis, and assays for oxidative stress and inflammatory markers were conducted. In vitro, SH-SY5Y, BV2, and GL261 cells were used to investigate the cytoprotective and mechanistic effects of THSG against Cd-induced neurotoxicity via WST-1, RT-qPCR, Western blotting, LDH release, and immunofl

ETHNOPHARMACOLOGICAL RELEVANCE: Hair plays an important roles in both local protection and information transmission. But as a widespread problem, the existing therapies of hair loss are limited. 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-Glucoside (Tetrahydroxy stilbene glucoside, TSG) is the main active compound of a hair promoting herb, Polygonum multiflorum Thunb., that has been used in China for over 1000 years. However, its role and mechanism of action remain unclear. AIM OF THE STUDY: To investigate the promoting effect of TSG on hair growth and elucidate the mechanism by which TSG affects hair regeneration via Th22. MATERIALS AND METHODS: Mouse skin explants and 3D ex vivo culture systems were employed to screen substances of P. multiflorum for promoting hair growth. The hair regeneration effect of TSG was explored via mice model by subcutaneous injection. The changes of biological dynamics, hair regeneration, histomorphology of skin, and morphology of hair follicle were observed. T cell subsets in peripheral blood was analyzed by Flow cytometry. Network pharmacology was used to predict the mechanism of TSG, immunohistochemistry and immunofluorescence staining were u

This study aims to research and discuss the molecular mechanism of Polygonum multiflorum (PM) to treat androgenic alopecia (APA). The target network and protein interaction network of APA of PM were established using traditional Chinese medicine molecular mechanism bioinformatics databases and disease gene databases. The core subnetworks were screened based on topological analysis, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed, molecular docking of the 10 genes with the highest connectivity. According to the intersection of active components and potential targets of PM, 1325 common target factors were obtained. The core subnetwork composed of 66 genes was screened by topological analysis. The results of Kyoto Encyclopedia of Genes and Genomes enrichment showed that PM mainly exerts biological functions by regulating inflammatory response, oxidation stress, apoptosis, autophagy, and other pathways. The result of molecular docking shows that the binding energies of HRAS, PIK3CA, PIK3CB, and RHOA -resveratrol, -polygonin, -chrysophanol, -rheum emodin, and -rhein were all <-9 kcal/mol. PM's mechanism in treating APA may regulate the expression of PIK3CA and PIK3CB through the emodin-mediated phosphoinositide 3-kinase/protein kinase B pathway to regulate cell apoptosis. In addition, some processes, such as inhibiting inflammation, oxidative stress, and inducing melanin production, are also involved. PM has application value in treating APA and can be popularized after testing.

This study examines the synergistic effects of extracts from Rhynchosia nulubilis (RN) and Polygonum multiflorum (PM) on the proliferation of human dermal papilla cells (hDPCs) and the alleviation of testosterone-induced cytotoxicity. Human dermal papilla cells (hDPCs) were treated with varying concentrations of RN and PM extracts, administered both individually and in multiple combinations at different ratios. The findings indicated that a 4:1 combination of RN and PM extracts significantly enhanced hDPC proliferation relative to the individual extracts, particularly in the presence of testosterone, which induced cytotoxicity. A significant synergistic effect was observed at a 4:1 ratio, resulting in the creation of a human growth factor array to identify targets associated with this synergy. The combined-extract group exhibited elevated levels of two significant growth factors: insulin-like growth factor-binding protein-1 (IGFBP-1) and neurotrophin-3 (NT-3

RATIONALE: Androgenic alopecia (AGA) is a prevalent condition with progressive miniaturization of hair follicles. Currently, reliable treatments have remained limited, and complementary medications for AGA are still being investigated. Traditional Chinese medicine formulas have conspicuous advantages in the treatment of AGA with good development prospects. Zimmer aqueous spray (ZAS) is a water spray containing Zimmer herbal extract powder (ZMWP), which consists of Ligustri lucidi Fructus, Ecliptae Herba, Fallopia multiflora (Thunb.) Harald. and Polygonatum sibiricum Delar. ex Redoute, etc. ZMWP is an active ingredient in the prevention of hair loss. Our aim is to provide evidence for the effectiveness of ZAS in the treatment of AGA. PATIENT CONCERNS: A 41-year-old man had suffered from hair loss for 8 years. INTERVENTIONS: The patient with moderate AGA received 3 to 4 mL ZAS daily or every other day for 3 months. OUTCOMES: The hair density obviously increased after 3 months of therapy. The improvement of hair diameter, vellus hair rate, and 1 hair pilosebaceous unit rate were observed with a trichoscopy and quantitatively analyzed. Besides, honeycomb pigment pattern mitigated and arborized red lines. LESSONS: The results suggested that ZMWP might have the capability of improving hair growth and attenuating AGA, which can be a promising alternative treatment of AGA.

Mesenchymal stem cell (MSC)-derived extracellular vesicles (exosomes) possess regeneration, cell proliferation, wound healing, and anti-senescence capabilities. The functions of exosomes can be modified by preconditioning MSCs through treatment with bio-pulsed reagents (Polygonum multiflorum Thunb extract). However, the beneficial effects of bio-pulsed small extracellular vesicles (sEVs) on the skin or hair remain unknown. This study investigated the in vitro mechanistic basis through which bio-pulsed sEVs enhance the bioactivity of the skin fibroblasts and hair follicle cells. Avian-derived MSCs (AMSCs) were isolated, characterized, and bio-pulsed to produce AMSC-sEVs, which were isolated, lyophilized, characterized, and analyzed. The effects of bio-pulsed AMSC-sEVs on cell proliferation, wound healing, and gene expression associated with skin and hair bioactivity were examined using human skin fibroblasts (HSFs) and follicle dermal papilla cells (HFDPCs). Bio-pulsed treatment significantly enhanced sEVs production by possibly upregulating RAB27A expression in AMSCs. Bio-pulsed AMSC-sEVs contained more exosomal proteins and RNAs than the control. Bio-pulsed AMSC-sEVs significantly augmented cell proliferation, wound healing, and gene expression in

BACKGROUND: Finasteride and minoxidil are two commonly used drugs for the treatment of hair loss. However, these two drugs have certain side effects. Thus, the further elucidation of treatments for hair loss, including those using Chinese herbal medicine, remains important clini